PtS2 nanosheets as a peroxidase-mimicking nanozyme for colorimetric determination of hydrogen peroxide and glucose

• Published in: Mikrochimica acta (1966) Vol. 188; no. 5; p. 174
• Main Authors: Zhang, Wenxian, Li, Xiuping, Cui, Tianyu, Li, Shenchang, Qian, Yuqing, Yue, Yu, Zhong, Wenying, Xu, Bo, Yue, Wanqing
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-05-2021; Springer Nature B.V
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Colorimetry; Dopamine; Electron transfer; Glucose; Glucose oxidase; Hyaluronic acid; Hydrogels; Hydrogen peroxide; Linearity; Microengineering; Microfluidics; Microspheres; Nanochemistry; Nanosheets; Nanotechnology; Original Paper; Oxidation; Peroxidase; Reproducibility; Sensors; Spectrophotometry; Microspheres; Nanozyme; Peroxidase-like activity; Glucose determination; PtS; nanosheets
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-021-04826-w

Using an ultrasonication-assisted liquid exfoliation method, we have synthesized PtS
2
nanosheets with good reproducibility. Herein, intrinsic peroxidase-like activity was for the first time demonstrated for PtS
2
nanosheets, which can catalyze H
2
O
2
oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to generate a colored solution. The catalytic mechanism of PtS
2
nanosheets was investigated, which indicated that acceleration of the electron transfer between TMB and H
2
O
2
was the main reason for the peroxidase-like activity of PtS
2
nanosheets. Based on these observations, we exploited PtS
2
nanosheets integrated into dopamine-functionalized hyaluronic acid (HA-DA) hydrogel microspheres by droplet microfluidics to construct PtS
2
nanosheet– and PtS
2
@HA-DA microsphere–based sensors for highly sensitive determination of H
2
O
2
. When coupled with glucose oxidase, we further developed two glucose sensors based on the above two methods. Among them, the linearity of the PtS
2
nanosheet–based spectrophotometry was in the range of 0.5 to 150 μM and the limit of detection as low as 0.20 μM. The linearity of the microsphere-based colorimetry was in the range 200 to 12,000 μM with a detection limit of 29.95 μM. Both of the glucose sensors can be applied to the determination of glucose in human serum with reliable results and reproducibility.
Graphical abstract