Evaluation and comparison of cytotoxicity and bioactivity of chemomechanical caries removal agents on stem cells from human exfoliated deciduous teeth

• Published in: European archives of paediatric dentistry Vol. 23; no. 5; pp. 787 - 796
• Main Authors: Maru, V., Madkaikar, M., Shabrish, S., Kambli, P., Dalvi, A., Setia, P.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-10-2022; Springer Nature B.V
• Subjects: Alizarin; Apoptosis; Biocompatibility; Biological activity; Calcium; Cytotoxicity; Dentin; Dentistry; Experiments; Flow cytometry; Gene expression; Medicine; Original Scientific Article; Pediatrics; Software; Stains & staining; Statistical analysis; Stem cells; Teeth; Toxicity; United States > US; Sweden; India; Cytotoxicity; SHED; Carisolv; Bioactivity; Papacarie
• ISSN: 1818-6300; 1996-9805
• DOI: 10.1007/s40368-021-00684-5

Aim
To investigate and compare the cytotoxicity and bioactivity of CMCR agents on stem cells derived from exfoliated deciduous teeth.
Methodology
MTT assay, flow cytometry, Alizarin Red staining and scratch assay were used to assess the cellular viability, apoptosis, calcium matrix deposits and cell migration, respectively. The gene expression of ALP and BMP-2 was measured with RT-PCR. One-way ANOVA and Bonferroni post-test was used for statistical analysis.
Results
0.5% Carisolv showed highest cell proliferation and calcium matrix formation, whereas 0.5% Papacarie reported the highest% live cells and cell migration. The highest mRNA expression of ALP and BMP-2 was reported in SHEDs cultured in 0.5% Papacarie (after 72 h incubation) and 0.5% Carisolv (after 24 h incubation), respectively.
Conclusion
CMCR agents are biocompatible and bioactive when cultured in stem cells derived from exfoliated primary teeth.