Enhanced production of prodigiosin by Serratia marcescens UCP 1549 using agrosubstrates in solid-state fermentation

• Published in: Archives of microbiology Vol. 203; no. 7; pp. 4091 - 4100
• Main Authors: dos Santos, Renata Andreia, Rodríguez, Dayana Montero, da Silva, Lucas Albuquerque Rosendo, de Almeida, Sérgio Mendonça, de Campos-Takaki, Galba Maria, de Lima, Marcos Antônio Barbosa
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-09-2021; Springer Nature B.V
• Subjects: Antiinfectives and antibacterials; Antimicrobial agents; Archives & records; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Ecology; Environmental impact; Fermentation; Functional groups; Infrared radiation; Life Sciences; Microbial Ecology; Microbiology; Original Paper; Pigments; Prodigiosin; Production costs; Saline solutions; Sodium chloride; Solid state; Solid state fermentation; Soybean oil; Soybeans; Substrates; Toxicity; Wheat; Wheat bran; Pigment; Static fermentation; Wheat bran
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-021-02399-z

This work aimed to investigate the production of prodigiosin by
S. marcescens
UCP 1549 in solid-state fermentation (SSF), as a sustainable alternative for reducing the production costs and environmental impact. Thus, different agro–industrial substrates were used in the formulation of the prodigiosin production medium, obtaining the maximum yield of pigment (119.8 g/kg dry substrate) in medium consisting of 5 g wheat bran, 5% waste soybean oil and saline solution. The pigment was confirmed as prodigiosin by the maximum absorbance peak at 535 nm, R
f
0.9 in TLC, and the functional groups by infrared spectrum (FTIR). Prodigiosin demonstrated stability at different values of temperature, pH and NaCl concentrations and antimicrobial properties, as well as not show any toxicity. These results confirm the applicability of SSF as a sustainable and promising technology and wheat bran as potential agrosubstrate to produce prodigiosin, making the bioprocess economic and competitive for industrial purposes.