Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential

Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential

Reports of non-neural differentiation of neural stem cells (NSCs) have been challenged by alternative explanations for expanded differentiation potentials. In an attempt to demonstrate the plasticity of NSC, neurospheres were generated from single retrovirally labeled embryonic cortical precursors....

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Published in:Development (Cambridge) Vol. 131; no. 17; pp. 4287 - 4298
Main Authors: Bani-Yaghoub, Mahmud, Kendall, Stephen E, Moore, Daniel P, Bellum, Stephen, Cowling, Rebecca A, Nikopoulos, George N, Kubu, Chris J, Vary, Calvin, Verdi, Joseph M
Format: Journal Article
Language:English
Published: England The Company of Biologists Limited 01-09-2004
Subjects:
Animals
Blotting, Southern
Blotting, Western
Cell Differentiation
Cell Line
Cell Lineage
Cell Survival
Cloning, Molecular
Coloring Agents - pharmacology
Culture Media, Serum-Free - pharmacology
DNA - metabolism
Dose-Response Relationship, Drug
Flow Cytometry
Immunohistochemistry
Insulin - metabolism
Insulin - physiology
Mice
Myocytes, Cardiac - metabolism
Neurons - cytology
Neurons - metabolism
Retroviridae - genetics
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - cytology
Time Factors
Transfection
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Abstract Reports of non-neural differentiation of neural stem cells (NSCs) have been challenged by alternative explanations for expanded differentiation potentials. In an attempt to demonstrate the plasticity of NSC, neurospheres were generated from single retrovirally labeled embryonic cortical precursors. In a defined serum-free insulin-containing media, 40% of the neurospheres contained both myogenic and neurogenic differentiated progeny. The number of NSCs displaying multilineage differentiation potential declines through gestation but does exist in the adult animal. In this system, insulin appears to function as a survival and dose-dependent myogenic differentiation signal for multilineage NSCs (MLNSC). MLNSC-derived cardiomyocytes contract synchronously, respond to sympathetic and parasympathetic stimulation, and regenerate injured heart tissues. These studies provide support for the hypothesis that MLNSCs exist throughout the lifetime of the animal, and potentially provide a population of stem cells for cell-based regenerative medicine strategies inside and outside of the nervous system.
AbstractList Reports of non-neural differentiation of neural stem cells (NSCs) have been challenged by alternative explanations for expanded differentiation potentials. In an attempt to demonstrate the plasticity of NSC, neurospheres were generated from single retrovirally labeled embryonic cortical precursors. In a defined serum-free insulin-containing media, 40% of the neurospheres contained both myogenic and neurogenic differentiated progeny. The number of NSCs displaying multilineage differentiation potential declines through gestation but does exist in the adult animal. In this system, insulin appears to function as a survival and dose-dependent myogenic differentiation signal for multilineage NSCs (MLNSC). MLNSC-derived cardiomyocytes contract synchronously, respond to sympathetic and parasympathetic stimulation, and regenerate injured heart tissues. These studies provide support for the hypothesis that MLNSCs exist throughout the lifetime of the animal, and potentially provide a population of stem cells for cell-based regenerative medicine strategies inside and outside of the nervous system.
Author Joseph M. Verdi
Calvin Vary
Chris J. Kubu
George N. Nikopoulos
Daniel P. Moore
Stephen E. Kendall
Stephen Bellum
Rebecca A. Cowling
Mahmud Bani-Yaghoub
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SubjectTerms Animals
Blotting, Southern
Blotting, Western
Cell Differentiation
Cell Line
Cell Lineage
Cell Survival
Cloning, Molecular
Coloring Agents - pharmacology
Culture Media, Serum-Free - pharmacology
DNA - metabolism
Dose-Response Relationship, Drug
Flow Cytometry
Immunohistochemistry
Insulin - metabolism
Insulin - physiology
Mice
Myocytes, Cardiac - metabolism
Neurons - cytology
Neurons - metabolism
Retroviridae - genetics
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - cytology
Time Factors
Transfection
Title Insulin acts as a myogenic differentiation signal for neural stem cells with multilineage differentiation potential
URI http://dev.biologists.org/content/131/17/4287.abstract
https://www.ncbi.nlm.nih.gov/pubmed/15294865
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