Purification of Rosmarinic Acid by Strong Ion-Exchange Centrifugal Partition Chromatography

• Published in: Journal of liquid chromatography & related technologies Vol. 28; no. 12-13; pp. 1947 - 1957
• Main Authors: Maciuk, Alexandre, Toribio, Alix, Zeches-Hanrot, Monique, Nuzillard, Jean-Marc, Renault, Jean-Hugues, Georgiev, Milen I., Ilieva, Mladenka P.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Colchester Taylor & Francis Group 01-07-2005; Taylor & Francis; Taylor & Francis: STM, Behavioural Science and Public Health Titles
• Subjects: Analytical chemistry; Biological and medical sciences; Centrifugal partition chromatography; Chemical Sciences; General pharmacology; Ion exchange; Medical sciences; Organic chemistry; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Purification; Rosmarinic acid; Countercurrent chromatography; Purification; Phase composition; Phenolic acid; Lavandula angustifolia; Pharmacognosy; Essential oil plant; Preparative chromatography; Medicinal plant; Labiatae; Dicotyledones; Cinnamic acid derivatives; Angiospermae; Plant origin; Ion exchanger; Isolation; Rosmarinic acid; Spermatophyta; Centrifugal partition chromatography; Ion exchange
• ISSN: 1082-6076; 1520-572X
• DOI: 10.1081/JLC-200063599

Ion-Exchange centrifugal partition chromatography using benzalkonium chloride as a strong exchanger (SIXCPC) was successfully used to purify rosmarinic acid from a crude extract that was produced by callus culture. The purification process was carried out on a gram scale using the ternary biphasic system CHCl
3
: n-BuOH∶water 4.5:1:4.5 v/v/v in the ascending mode (mobile aqueous phase and stationary organic phase). Two particular points are discussed: the influence of benzalkonium chloride on ternary solvent system stability and the advantage of injecting the analytes as sodium salts rather than molecular acids.