Environmental pH affects photoautotrophic growth of Synechocystis sp. PCC 6803 strains carrying mutations in the lumenal proteins of PSII

• Published in: Plant and cell physiology Vol. 54; no. 6; pp. 859 - 874
• Main Authors: Summerfield, Tina C, Crawford, Tim S, Young, Roger D, Chua, Jocelyn P S, Macdonald, Rebecca L, Sherman, Louis A, Eaton-Rye, Julian J
• Format: Journal Article
• Language: English
• Published: Japan 01-06-2013
• Subjects: Autotrophic Processes - drug effects; Autotrophic Processes - radiation effects; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Buffers; Chlorophyll - metabolism; Culture Media - pharmacology; Environment; Gene Expression Regulation, Bacterial - drug effects; Gene Expression Regulation, Bacterial - radiation effects; Genes, Bacterial - genetics; Heterotrophic Processes - drug effects; Heterotrophic Processes - radiation effects; Hydrogen-Ion Concentration - drug effects; Kinetics; Models, Biological; Mutation - genetics; Oxidative Stress - drug effects; Oxidative Stress - genetics; Oxidative Stress - radiation effects; Photosystem II Protein Complex - genetics; Photosystem II Protein Complex - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rose Bengal - pharmacology; Singlet Oxygen - pharmacology; Spectrometry, Fluorescence; Synechocystis - drug effects; Synechocystis - genetics; Synechocystis - growth & development; Synechocystis - metabolism; Transcription, Genetic - drug effects; Transcription, Genetic - radiation effects; Alkaline pH; Oxidative stress; Gene expression; Photosynthesis; Synechocystis sp. PCC 6803; PSII extrinsic proteins
• DOI: 10.1093/pcp/pct036
• EISSN: 1471-9053
• ISSN: 0032-0781

Synechocystis sp. strain PCC 6803 grows photoautotrophically across a broad pH range, but wild-type cultures reach a higher density at elevated pH; however, photoheterotrophic growth is similar at high and neutral pH. A number of PSII mutants each lacking at least one lumenal extrinsic protein, and carrying a second PSII lumenal mutation, are able to grow photoautotrophically in BG-11 medium at pH 10.0, but not pH 7.5. We investigated the basis of this pH effect and observed no pH-specific change in variable fluorescence yield from PSII centers of the wild type or the pH-dependent ΔPsbO:ΔPsbU and ΔPsbV:ΔCyanoQ strains; however, 77 K fluorescence emission spectra indicated increased coupling of the phycobilisome (PBS) antenna at pH 10.0 in all mutants. DNA microarray data showed a cell-wide response to transfer from pH 10.0 to pH 7.5, including decreased mRNA levels of a number of oxidative stress-responsive transcripts. We hypothesize that this transcriptional response led to increased tolerance against reactive oxygen species and in particular singlet oxygen. This response enabled photoautotrophic growth of the PSII mutants at pH 10.0. This hypothesis was supported by increased resistance of all strains to rose bengal at pH 10.0 compared with pH 7.5.