Defocusing microscopy: An approach for red blood cell optics
Thin transparent objects (phase objects) can become visible in a bright-field light microscope, if the microscope is slightly defocused. Thick transparent objects, like red blood cells (RBC), are seen because some of their parts are always out of focus. By applying our recently developed defocusing...
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| Published in: | Applied physics letters Vol. 88; no. 13; pp. 133901 - 133901-3 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
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American Institute of Physics
27-03-2006
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| Abstract | Thin transparent objects (phase objects) can become visible in a bright-field light microscope, if the microscope is slightly defocused. Thick transparent objects, like red blood cells (RBC), are seen because some of their parts are always out of focus. By applying our recently developed defocusing microscopy technique to RBC, we are able to interpret RBC bright-field light microscopy images, an old standing problem. From the average image contrast we obtain RBC shape, size, and refractive index. From contrast fluctuations caused by the flicker phenomenon, we obtain RBC bending modulus and cytoplasm viscosity. |
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| AbstractList | Thin transparent objects (phase objects) can become visible in a bright-field light microscope, if the microscope is slightly defocused. Thick transparent objects, like red blood cells (RBC), are seen because some of their parts are always out of focus. By applying our recently developed defocusing microscopy technique to RBC, we are able to interpret RBC bright-field light microscopy images, an old standing problem. From the average image contrast we obtain RBC shape, size, and refractive index. From contrast fluctuations caused by the flicker phenomenon, we obtain RBC bending modulus and cytoplasm viscosity. |
| Author | Agero, Ubirajara Mesquita, Leonardo G. Mesquita, Oscar N. |
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| Cites_doi | 10.1103/PhysRevE.67.051904 10.1002/jemt.20117 10.1016/0026-2862(72)90069-6 10.1103/PhysRevLett.92.018102 10.1103/PhysRevA.45.4116 10.1073/pnas.94.10.5045 10.1007/s002490000122 10.1529/biophysj.104.045328 10.1016/S0006-3495(95)79921-0 10.1016/S0006-3495(83)84319-7 10.1016/j.yexcr.2004.09.002 10.1051/jphys:0197500360110103500 10.1051/jphys:0198700480120213900 |
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| References | Tuvia, S.; Almagor, A.; Bitler, A.; Levin, S.; Korenstein, R.; Yedgar, S. 1997; 94 Agero, U.; Mesquita, L.; Neves, B.; Gazzinelli, R.; Mesquita, O. 2004; 65 Strey, H.; Peterson, M.; Sackmann, E. 1995; 69 Peterson, M. 1992; 45 Evans, E.; Fung, Y-C. 1972; 4 Agero, U.; Monken, C.; Ropert, C.; Gazzinelli, R.; Mesquita, O. 2003; 67 Zilker, A.; Engelhardt, H.; Sackmann, E. 1987; 48 Evans, E. 1983; 43 Lacoste, D.; Raphael, E. 2004; 92 Brochard, F.; Lennon, J. 1975; 36 Scheffer, L.; Bitler, A.; Ben-Jacob, E.; Korenstein, R. 2001; 30 Coelho Neto, J.; Agero, U.; Oliveira, D.; Gazzinelli, R.; Mesquita, O. 2005; 303 Gov, N.; Safran, S. 2005; 88 (2023070301203523700_c1) 2003; 67 (2023070301203523700_c6) 1987; 48 (2023070301203523700_c7) 1995; 69 (2023070301203523700_c8) 2001; 30 (2023070301203523700_c9) 1975; 36 (2023070301203523700_c4) 1972; 4 (2023070301203523700_c12) 1997; 94 (2023070301203523700_c13) 1992; 45 (2023070301203523700_c3) 2005; 303 (2023070301203523700_c5) 1983; 43 (2023070301203523700_c10) 2005; 88 (2023070301203523700_c11) 2004; 92 (2023070301203523700_c2) 2004; 65 |
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| Title | Defocusing microscopy: An approach for red blood cell optics |
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