Microbiome and response surface methodology analyses reveal Acetobacter pasteurianus as the core bacteria responsible for aerobic spoilage of corn silage ( Zea mays ) in hot and humid areas

• Published in: Frontiers in microbiology Vol. 15; p. 1473238
• Main Authors: Bai, Rui, Li, Haiping, Chen, Shiyong, Yuan, Xianjun, Chen, Youjun, Huang, Yanling, Zhou, Qingping, Guan, Hao
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 11-09-2024
• Subjects: Acetobacter pasteurianus; aerobic stability; bacterial community; Microbiology; response surface methodology; whole-plant corn silage; aerobic stability; bacterial community; response surface methodology; Acetobacter pasteurianus; whole-plant corn silage
• DOI: 10.3389/fmicb.2024.1473238
• EISSN: 1664-302X

Weak aerobic stability is a notable challenge for whole-plant corn silage, particularly in hot and humid regions.
is commonly regarded as an indicator of aerobic deterioration in silage, yet its precise role in fermentation and during aerobic exposure, as well as the factors that promote its growth, remain insufficiently understood.
In this study, whole-plant corn silage was prepared using a bagged method with controlled dry matter (DM) content at 20%, 25%, and 30%, and initial concentrations of
at 40%, 50%, and 60%. The silage was stored for 60 days under varying temperatures (20°C, 30°C, and 40°C). Following the anaerobic storage phase, the silage was exposed to air at room temperature (20-25°C) for 7 days, both with and without
inoculation.
The results demonstrated that
did not impact the nutritional value of the silage during anaerobic fermentation, maintaining a low pH (< 3.80). However, during aerobic exposure, the presence of
significantly reduced the aerobic stability of the silage. The microbial community shifted from primarily
species initially to
and
species post-ensiling. During the aerobic exposure phase,
and
became the dominant species. Response Surface Methodology (RSM) analysis identified optimal conditions for the proliferation of
during the aerobic phase, which occurred at 28°C, 25% DM, and 52% initial concentration at 3 ml/kg.
These findings confirm that
plays a critical role in reducing the aerobic stability of whole-plant corn silage. Additionally, the study identifies the optimal conditions that favor the proliferation of
, offering valuable insights for the development of strategies to prevent and control this bacterium, thereby improving the aerobic stability of silage in hot and humid regions.