Sodium dodecyl sulfate polyacrylamide gel electrophoresis for direct quantitation of protein adsorption

A simple method, sodium dodecyl sulfate polyacrylamide gel electrophoresis coupled with direct protein adsorption analysis (SDS–PAGE/DPA), is presented here for the quantitation of adsorption-caused protein loss. No complicated steps and expensive equipment are involved, and this method is capable o...

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Bibliographic Details
Published in:	Analytical biochemistry Vol. 465; pp. 102 - 104
Main Authors:	Lee, Man Chung Gilbert, Wu, Kinny Sheung Yang, Nguyen, Tam N.T., Sun, Bingyun
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15-11-2014
Subjects:	Adsorption Animals Cattle Electrophoresis, Polyacrylamide Gel - methods Protein adsorption Sample loss SDS–PAGE SDS–PAGE/DPA Sensitive protein analysis Serum Albumin, Bovine - chemistry Single-cell proteomics Single-cell proteomics Sample loss SDS–PAGE SDS–PAGE/DPA Protein adsorption Sensitive protein analysis
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Summary:	A simple method, sodium dodecyl sulfate polyacrylamide gel electrophoresis coupled with direct protein adsorption analysis (SDS–PAGE/DPA), is presented here for the quantitation of adsorption-caused protein loss. No complicated steps and expensive equipment are involved, and this method is capable of measuring proteins adsorbed on sample vials at extremely low concentrations (in pg/μl). We used this method to characterize the effects of concentration, time, and volume on adsorption. We also applied this method to discover differential sample loss in protein mixtures and its utility in developing preventive strategies of adsorption.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0003-2697 1096-0309
DOI:	10.1016/j.ab.2014.07.031