RAPD Marker Polymorphism among Saskatoon Cultivars, Clones, and Seedlings

• Published in: HortScience Vol. 32; no. 6; pp. 1109 - 1113
• Main Authors: Weir, B.J, Pierre, R.G. St, Chibbar, R.N
• Format: Journal Article
• Language: English
• Published: Alexandria, VA American Society for Horticultural Science 01-10-1997
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; Genetic resources, diversity; Genetics and breeding of economic plants; Plant material; Canada; North America; America; Genetic variability; Ornamental shrub; Genetic marker; Varietal identification; Plant juvenile growth stage; Rosaceae; Polymerase chain reaction; Random amplified polymorphic DNA; Amelanchier alnifolia; Dicotyledones; Angiospermae; Spermatophyta; Clone; Cultivar
• ISSN: 0018-5345; 2327-9834
• DOI: 10.21273/hortsci.32.6.1109

Randomly amplified polymorphic DNA (RAPD) markers were used to distinguish among 16 cultivars of saskatoon ( Amelanchier spp.). Eight 9-base, oligonucleotide primers amplified a total of 98 DNA fragments, of which 29 were useful as reproducible polymorphic markers. Twelve cultivars and two pairs of cultivars were uniquely characterized by these 29 markers. Polymorphism was not detected among five sources of the cv. Thiessen, whereas variability was found among seedlings from self-pollinated `Thiessen'. Samples of the cvs. Regent and Parkhill were indistinguishable from one of two sources, suggesting that the cultivars were mislabelled.