Construction and use of PCR primers from a 65 kDa mannoprotein gene for identification of C. albicans

• Published in: Molecular and cellular probes Vol. 18; no. 3; pp. 171 - 175
• Main Authors: Arancia, S, Sandini, S, Cassone, A, De Bernardis, F, La Valle, R
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-06-2004
• Subjects: Blotting, Southern; CaMP65; Candida albicans; Candida albicans - genetics; Candida albicans - isolation & purification; Candidiasis - microbiology; DNA Primers - genetics; DNA, Fungal - analysis; DNA, Fungal - genetics; Genes, Fungal - genetics; Humans; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Molecular diagnosis; Molecular Weight; Mycological Typing Techniques - methods; Polymerase chain reaction; Polymerase Chain Reaction - methods; Species Specificity; Candida albicans; Polymerase chain reaction; Molecular diagnosis; CaMP65
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1016/j.mcp.2003.11.005

A method for detection of
Candida albicans in biological samples (blood, serum, urine) was developed by the use of polymerase chain reaction (PCR) amplification of a DNA fragment of a gene coding for a 65 kDa mannoprotein of
C. albicans (CaMP65). The PCR amplifies a 220 bp fragments whose specificity for
C. albicans was demonstrated by Southern blot with a non-radioactive probe, leading to the differentiation from all other yeast species or human and bacterial DNA. The sensitivity of this assay was 5–10
C. albicans cells per milliliter of biological sample.