Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil

Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin...

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Published in:Ciência rural Vol. 45; no. 9; pp. 1619 - 1621
Main Authors: Silva, Rodrigo Otávio Silveira, Gabardo, Michelle de Paula, Oliveira, Juliana Saes Vilaça de, Lobato, Francisco Carlos Faria, Guedes, Roberto Maurício Carvalho
Format: Journal Article
Language:English
Published: Universidade Federal de Santa Maria 01-09-2015
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Abstract Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularisé uma bactéria intracelular obrigatória responsável pela enteropatia proliferativa, uma doença entérica endêmica em suínos e comum em potros. Em cães, no entanto, existem poucos estudos sobre essa doença e inexistem relatos de cães infectados por L. intracellularisna América Latina. O objetivo deste estudo foi avaliar a presença de L. intracellularisem amostras de fezes de cães diarréicos e aparentemente saudáveis em Minas Gerais, Brasil. Foram incluídos 58 cães, sendo 18 aparentemente saudáveis e 40 diarréicos. Submeteu-se o DNA bacteriano fecal a uma reação de PCR nested para L. intracellularis. Três das 40 (7,5%) amostras de cães diarréicos foram positivas para L. intracellularis, estando todos esses animais com aproximadamente 3 meses de idade. Estes resultados salientam a necessidade de mais estudos para confirmar o papel deste patógeno como uma possível causa de doença entérica em cães
AbstractList Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs
Lawsonia intracellularis is an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularis in Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularis in diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis. Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis . These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs
Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularisé uma bactéria intracelular obrigatória responsável pela enteropatia proliferativa, uma doença entérica endêmica em suínos e comum em potros. Em cães, no entanto, existem poucos estudos sobre essa doença e inexistem relatos de cães infectados por L. intracellularisna América Latina. O objetivo deste estudo foi avaliar a presença de L. intracellularisem amostras de fezes de cães diarréicos e aparentemente saudáveis em Minas Gerais, Brasil. Foram incluídos 58 cães, sendo 18 aparentemente saudáveis e 40 diarréicos. Submeteu-se o DNA bacteriano fecal a uma reação de PCR nested para L. intracellularis. Três das 40 (7,5%) amostras de cães diarréicos foram positivas para L. intracellularis, estando todos esses animais com aproximadamente 3 meses de idade. Estes resultados salientam a necessidade de mais estudos para confirmar o papel deste patógeno como uma possível causa de doença entérica em cães
Author Oliveira, Juliana Saes Vilaça de
Guedes, Roberto Maurício Carvalho
Silva, Rodrigo Otávio Silveira
Lobato, Francisco Carlos Faria
Gabardo, Michelle de Paula
AuthorAffiliation Universidade Federal de Minas Gerais
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  givenname: Michelle de Paula
  surname: Gabardo
  fullname: Gabardo, Michelle de Paula
  organization: Universidade Federal de Minas Gerais, Brazil
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  organization: Universidade Federal de Minas Gerais, Brazil
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  givenname: Francisco Carlos Faria
  surname: Lobato
  fullname: Lobato, Francisco Carlos Faria
  organization: Universidade Federal de Minas Gerais, Brazil
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  givenname: Roberto Maurício Carvalho
  surname: Guedes
  fullname: Guedes, Roberto Maurício Carvalho
  organization: Universidade Federal de Minas Gerais, Brazil
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10.1053/jcpa.1999.0347
10.1177/030098589303000413
10.1128/JCM.31.10.2611-2615.1993
10.17221/5757-VETMED
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DocumentTitleAlternate Detecção de Lawsonia intracellularis em fezes de cães em Minas Gerais, Brasil
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Issue 9
Keywords canídeos
diarreia
Lawsonia intracellularis
canine
diarrhea
enteropatia proliferativa
proliferative enteropathy
Language English
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References LEBLANC B. (ref8) 1993; 30
TOMANOVÁ K. (ref12) 2003; 48
HÚSNIK R. (ref4) 2008; 48
JONES G.F. (ref5) 1993; 31
MACÊDO N.R. (ref9) 2007; 59
SILVA R.O.S (ref11) 2013; 45
MCORIST S. (ref10) 1995; 45
COLLINS J.E. (ref1) 1983; 183
LAWSON G.H.K. (ref7) 2000; 122
KLIMES J. (ref6) 2007; 52
HERBST W. (ref2) 2003; 110
HERRING A.J. (ref3) 1982; 16
VANNUCCI F.A. (ref13) 2014; 51
1982; 16
1983; 183
2013; 45
1995; 45
1993; 31
1993; 30
2008; 48
2003; 48
2000; 122
2007; 52
2014; 51
2003; 110
2007; 59
References_xml – volume: 183
  start-page: 886
  year: 1983
  ident: ref1
  article-title: Proliferative enteritis in two pups
  publication-title: Journal of the American Veterinary Medical Association
  contributor:
    fullname: COLLINS J.E.
– volume: 59
  start-page: 1117
  year: 2007
  ident: ref9
  article-title: Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarreicos
  publication-title: Arquivo Brasileiro de Medicina Veterinária e Zooctenia
  doi: 10.1590/S0102-09352007000500005
  contributor:
    fullname: MACÊDO N.R.
– volume: 45
  start-page: 671
  year: 2013
  ident: ref11
  article-title: Detection of A/B toxin and isolation of Clostridium difficile and C. perfringens from foals
  publication-title: Equine Veterinary Journal
  doi: 10.1111/evj.12046
  contributor:
    fullname: SILVA R.O.S
– volume: 48
  start-page: 141
  year: 2008
  ident: ref4
  article-title: Lawsonia intracellularis in a dog with inflammatory bowel disease
  publication-title: Veterinarni Medicina
  doi: 10.17221/5761-VETMED
  contributor:
    fullname: HÚSNIK R.
– volume: 16
  start-page: 473
  year: 1982
  ident: ref3
  article-title: Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels
  publication-title: Journal of Clinical Microbiology
  doi: 10.1128/JCM.16.3.473-477.1982
  contributor:
    fullname: HERRING A.J.
– volume: 52
  start-page: 502
  year: 2007
  ident: ref6
  article-title: Prevalence of antibodies against Lawsonia intracellularis in dogs with and without gastrointestinal disease
  publication-title: Veterinari Medicina
  doi: 10.17221/2062-VETMED
  contributor:
    fullname: KLIMES J.
– volume: 45
  start-page: 820
  year: 1995
  ident: ref10
  article-title: Characterization of Lawsonia intracellularis gen. nov, sp nov, the obligately intracellular bacterium of porcine proliferative enteropathy
  publication-title: International Journal of Systematic Bacteriology
  doi: 10.1099/00207713-45-4-820
  contributor:
    fullname: MCORIST S.
– volume: 122
  start-page: 77
  year: 2000
  ident: ref7
  article-title: Proliferative enteropathy: review
  publication-title: Journal of Comparative Pathology
  doi: 10.1053/jcpa.1999.0347
  contributor:
    fullname: LAWSON G.H.K.
– volume: 30
  start-page: 391
  year: 1993
  ident: ref8
  article-title: Hyperplastic gastritis with intraepithelial Campylobacter-like organisms in a Beagle dog
  publication-title: Veterinary Pathology
  doi: 10.1177/030098589303000413
  contributor:
    fullname: LEBLANC B.
– volume: 51
  start-page: 465
  year: 2014
  ident: ref13
  article-title: Recent advances in understanding the pathogenesis of Lawsonia intracellularis infections
  publication-title: Veteterinary Pathology
  contributor:
    fullname: VANNUCCI F.A.
– volume: 31
  start-page: 2611
  year: 1993
  ident: ref5
  article-title: Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction
  publication-title: Journal of Clinical Microbiology
  doi: 10.1128/JCM.31.10.2611-2615.1993
  contributor:
    fullname: JONES G.F.
– volume: 110
  start-page: 361
  year: 2003
  ident: ref2
  article-title: Diagnosis of Lawsonia intracellularis using the polymerase chain reaction (PCR) in pigs with and without diarrhea and other animal species
  publication-title: Dtsch Tierarztl Wochenschr
  contributor:
    fullname: HERBST W.
– volume: 48
  start-page: 108
  year: 2003
  ident: ref12
  article-title: Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology
  publication-title: Veterinarni Medicina
  doi: 10.17221/5757-VETMED
  contributor:
    fullname: TOMANOVÁ K.
– volume: 48
  start-page: 141
  year: 2008
  end-page: 145
  article-title: Lawsonia intracellularis in a dog with inflammatory bowel disease
  publication-title: Veterinarni Medicina
– volume: 45
  start-page: 820
  year: 1995
  end-page: 825
  article-title: Characterization of Lawsonia intracellularis gen. nov, sp nov, the obligately intracellular bacterium of porcine proliferative enteropathy
  publication-title: International Journal of Systematic Bacteriology
– volume: 183
  start-page: 886
  year: 1983
  end-page: 889
  article-title: Proliferative enteritis in two pups
  publication-title: Journal of the American Veterinary Medical Association
– volume: 51
  start-page: 465
  year: 2014
  end-page: 477
  article-title: Recent advances in understanding the pathogenesis of Lawsonia intracellularis infections
  publication-title: Veteterinary Pathology
– volume: 16
  start-page: 473
  year: 1982
  end-page: 477
  article-title: Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels
  publication-title: Journal of Clinical Microbiology
– volume: 48
  start-page: 108
  year: 2003
  end-page: 112
  article-title: Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology
  publication-title: Veterinarni Medicina
– volume: 31
  start-page: 2611
  year: 1993
  end-page: 2615
  article-title: Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction
  publication-title: Journal of Clinical Microbiology
– volume: 122
  start-page: 77
  year: 2000
  end-page: 100
  article-title: Proliferative enteropathy: review
  publication-title: Journal of Comparative Pathology
– volume: 59
  start-page: 1117
  year: 2007
  end-page: 1123
  article-title: Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarreicos
  publication-title: Arquivo Brasileiro de Medicina Veterinária e Zooctenia
– volume: 45
  start-page: 671
  year: 2013
  end-page: 675
  article-title: Detection of A/B toxin and isolation of Clostridium difficile and C. perfringens from foals
  publication-title: Equine Veterinary Journal
– volume: 52
  start-page: 502
  year: 2007
  end-page: 506
  article-title: Prevalence of antibodies against Lawsonia intracellularis in dogs with and without gastrointestinal disease
  publication-title: Veterinari Medicina
– volume: 30
  start-page: 391
  year: 1993
  end-page: 394
  article-title: Hyperplastic gastritis with intraepithelial Campylobacter-like organisms in a Beagle dog
  publication-title: Veterinary Pathology
– volume: 110
  start-page: 361
  year: 2003
  end-page: 364
  article-title: Diagnosis of Lawsonia intracellularis using the polymerase chain reaction (PCR) in pigs with and without diarrhea and other animal species
  publication-title: Dtsch Tierarztl Wochenschr
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Snippet Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and...
Lawsonia intracellularis is an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and...
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SubjectTerms AGRONOMY
canídeos
diarreia
enteropatia proliferativa
Lawsonia intracellularis
Title Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil
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