Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil
Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin...
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Published in: | Ciência rural Vol. 45; no. 9; pp. 1619 - 1621 |
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Abstract | Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs
Lawsonia intracellularisé uma bactéria intracelular obrigatória responsável pela enteropatia proliferativa, uma doença entérica endêmica em suínos e comum em potros. Em cães, no entanto, existem poucos estudos sobre essa doença e inexistem relatos de cães infectados por L. intracellularisna América Latina. O objetivo deste estudo foi avaliar a presença de L. intracellularisem amostras de fezes de cães diarréicos e aparentemente saudáveis em Minas Gerais, Brasil. Foram incluídos 58 cães, sendo 18 aparentemente saudáveis e 40 diarréicos. Submeteu-se o DNA bacteriano fecal a uma reação de PCR nested para L. intracellularis. Três das 40 (7,5%) amostras de cães diarréicos foram positivas para L. intracellularis, estando todos esses animais com aproximadamente 3 meses de idade. Estes resultados salientam a necessidade de mais estudos para confirmar o papel deste patógeno como uma possível causa de doença entérica em cães |
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AbstractList | Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularis is an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularis in Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularis in diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis. Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis . These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularisé uma bactéria intracelular obrigatória responsável pela enteropatia proliferativa, uma doença entérica endêmica em suínos e comum em potros. Em cães, no entanto, existem poucos estudos sobre essa doença e inexistem relatos de cães infectados por L. intracellularisna América Latina. O objetivo deste estudo foi avaliar a presença de L. intracellularisem amostras de fezes de cães diarréicos e aparentemente saudáveis em Minas Gerais, Brasil. Foram incluídos 58 cães, sendo 18 aparentemente saudáveis e 40 diarréicos. Submeteu-se o DNA bacteriano fecal a uma reação de PCR nested para L. intracellularis. Três das 40 (7,5%) amostras de cães diarréicos foram positivas para L. intracellularis, estando todos esses animais com aproximadamente 3 meses de idade. Estes resultados salientam a necessidade de mais estudos para confirmar o papel deste patógeno como uma possível causa de doença entérica em cães |
Author | Oliveira, Juliana Saes Vilaça de Guedes, Roberto Maurício Carvalho Silva, Rodrigo Otávio Silveira Lobato, Francisco Carlos Faria Gabardo, Michelle de Paula |
AuthorAffiliation | Universidade Federal de Minas Gerais |
AuthorAffiliation_xml | – name: Universidade Federal de Minas Gerais |
Author_xml | – sequence: 1 givenname: Rodrigo Otávio Silveira surname: Silva fullname: Silva, Rodrigo Otávio Silveira organization: Universidade Federal de Minas Gerais, Brazil – sequence: 2 givenname: Michelle de Paula surname: Gabardo fullname: Gabardo, Michelle de Paula organization: Universidade Federal de Minas Gerais, Brazil – sequence: 3 givenname: Juliana Saes Vilaça de surname: Oliveira fullname: Oliveira, Juliana Saes Vilaça de organization: Universidade Federal de Minas Gerais, Brazil – sequence: 4 givenname: Francisco Carlos Faria surname: Lobato fullname: Lobato, Francisco Carlos Faria organization: Universidade Federal de Minas Gerais, Brazil – sequence: 5 givenname: Roberto Maurício Carvalho surname: Guedes fullname: Guedes, Roberto Maurício Carvalho organization: Universidade Federal de Minas Gerais, Brazil |
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Cites_doi | 10.1590/S0102-09352007000500005 10.1111/evj.12046 10.17221/5761-VETMED 10.1128/JCM.16.3.473-477.1982 10.17221/2062-VETMED 10.1099/00207713-45-4-820 10.1053/jcpa.1999.0347 10.1177/030098589303000413 10.1128/JCM.31.10.2611-2615.1993 10.17221/5757-VETMED |
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DocumentTitleAlternate | Detecção de Lawsonia intracellularis em fezes de cães em Minas Gerais, Brasil |
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Keywords | canídeos diarreia Lawsonia intracellularis canine diarrhea enteropatia proliferativa proliferative enteropathy |
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References | LEBLANC B. (ref8) 1993; 30 TOMANOVÁ K. (ref12) 2003; 48 HÚSNIK R. (ref4) 2008; 48 JONES G.F. (ref5) 1993; 31 MACÊDO N.R. (ref9) 2007; 59 SILVA R.O.S (ref11) 2013; 45 MCORIST S. (ref10) 1995; 45 COLLINS J.E. (ref1) 1983; 183 LAWSON G.H.K. (ref7) 2000; 122 KLIMES J. (ref6) 2007; 52 HERBST W. (ref2) 2003; 110 HERRING A.J. (ref3) 1982; 16 VANNUCCI F.A. (ref13) 2014; 51 1982; 16 1983; 183 2013; 45 1995; 45 1993; 31 1993; 30 2008; 48 2003; 48 2000; 122 2007; 52 2014; 51 2003; 110 2007; 59 |
References_xml | – volume: 183 start-page: 886 year: 1983 ident: ref1 article-title: Proliferative enteritis in two pups publication-title: Journal of the American Veterinary Medical Association contributor: fullname: COLLINS J.E. – volume: 59 start-page: 1117 year: 2007 ident: ref9 article-title: Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarreicos publication-title: Arquivo Brasileiro de Medicina Veterinária e Zooctenia doi: 10.1590/S0102-09352007000500005 contributor: fullname: MACÊDO N.R. – volume: 45 start-page: 671 year: 2013 ident: ref11 article-title: Detection of A/B toxin and isolation of Clostridium difficile and C. perfringens from foals publication-title: Equine Veterinary Journal doi: 10.1111/evj.12046 contributor: fullname: SILVA R.O.S – volume: 48 start-page: 141 year: 2008 ident: ref4 article-title: Lawsonia intracellularis in a dog with inflammatory bowel disease publication-title: Veterinarni Medicina doi: 10.17221/5761-VETMED contributor: fullname: HÚSNIK R. – volume: 16 start-page: 473 year: 1982 ident: ref3 article-title: Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels publication-title: Journal of Clinical Microbiology doi: 10.1128/JCM.16.3.473-477.1982 contributor: fullname: HERRING A.J. – volume: 52 start-page: 502 year: 2007 ident: ref6 article-title: Prevalence of antibodies against Lawsonia intracellularis in dogs with and without gastrointestinal disease publication-title: Veterinari Medicina doi: 10.17221/2062-VETMED contributor: fullname: KLIMES J. – volume: 45 start-page: 820 year: 1995 ident: ref10 article-title: Characterization of Lawsonia intracellularis gen. nov, sp nov, the obligately intracellular bacterium of porcine proliferative enteropathy publication-title: International Journal of Systematic Bacteriology doi: 10.1099/00207713-45-4-820 contributor: fullname: MCORIST S. – volume: 122 start-page: 77 year: 2000 ident: ref7 article-title: Proliferative enteropathy: review publication-title: Journal of Comparative Pathology doi: 10.1053/jcpa.1999.0347 contributor: fullname: LAWSON G.H.K. – volume: 30 start-page: 391 year: 1993 ident: ref8 article-title: Hyperplastic gastritis with intraepithelial Campylobacter-like organisms in a Beagle dog publication-title: Veterinary Pathology doi: 10.1177/030098589303000413 contributor: fullname: LEBLANC B. – volume: 51 start-page: 465 year: 2014 ident: ref13 article-title: Recent advances in understanding the pathogenesis of Lawsonia intracellularis infections publication-title: Veteterinary Pathology contributor: fullname: VANNUCCI F.A. – volume: 31 start-page: 2611 year: 1993 ident: ref5 article-title: Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction publication-title: Journal of Clinical Microbiology doi: 10.1128/JCM.31.10.2611-2615.1993 contributor: fullname: JONES G.F. – volume: 110 start-page: 361 year: 2003 ident: ref2 article-title: Diagnosis of Lawsonia intracellularis using the polymerase chain reaction (PCR) in pigs with and without diarrhea and other animal species publication-title: Dtsch Tierarztl Wochenschr contributor: fullname: HERBST W. – volume: 48 start-page: 108 year: 2003 ident: ref12 article-title: Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology publication-title: Veterinarni Medicina doi: 10.17221/5757-VETMED contributor: fullname: TOMANOVÁ K. – volume: 48 start-page: 141 year: 2008 end-page: 145 article-title: Lawsonia intracellularis in a dog with inflammatory bowel disease publication-title: Veterinarni Medicina – volume: 45 start-page: 820 year: 1995 end-page: 825 article-title: Characterization of Lawsonia intracellularis gen. nov, sp nov, the obligately intracellular bacterium of porcine proliferative enteropathy publication-title: International Journal of Systematic Bacteriology – volume: 183 start-page: 886 year: 1983 end-page: 889 article-title: Proliferative enteritis in two pups publication-title: Journal of the American Veterinary Medical Association – volume: 51 start-page: 465 year: 2014 end-page: 477 article-title: Recent advances in understanding the pathogenesis of Lawsonia intracellularis infections publication-title: Veteterinary Pathology – volume: 16 start-page: 473 year: 1982 end-page: 477 article-title: Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels publication-title: Journal of Clinical Microbiology – volume: 48 start-page: 108 year: 2003 end-page: 112 article-title: Detection of Lawsonia intracellularis in a dog with inflammatory bowel disease using nested PCR and serology publication-title: Veterinarni Medicina – volume: 31 start-page: 2611 year: 1993 end-page: 2615 article-title: Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction publication-title: Journal of Clinical Microbiology – volume: 122 start-page: 77 year: 2000 end-page: 100 article-title: Proliferative enteropathy: review publication-title: Journal of Comparative Pathology – volume: 59 start-page: 1117 year: 2007 end-page: 1123 article-title: Detecção de cepas patogênicas pela PCR multiplex e avaliação da sensibilidade a antimicrobianos de Escherichia coli isoladas de leitões diarreicos publication-title: Arquivo Brasileiro de Medicina Veterinária e Zooctenia – volume: 45 start-page: 671 year: 2013 end-page: 675 article-title: Detection of A/B toxin and isolation of Clostridium difficile and C. perfringens from foals publication-title: Equine Veterinary Journal – volume: 52 start-page: 502 year: 2007 end-page: 506 article-title: Prevalence of antibodies against Lawsonia intracellularis in dogs with and without gastrointestinal disease publication-title: Veterinari Medicina – volume: 30 start-page: 391 year: 1993 end-page: 394 article-title: Hyperplastic gastritis with intraepithelial Campylobacter-like organisms in a Beagle dog publication-title: Veterinary Pathology – volume: 110 start-page: 361 year: 2003 end-page: 364 article-title: Diagnosis of Lawsonia intracellularis using the polymerase chain reaction (PCR) in pigs with and without diarrhea and other animal species publication-title: Dtsch Tierarztl Wochenschr |
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SubjectTerms | AGRONOMY canídeos diarreia enteropatia proliferativa Lawsonia intracellularis |
Title | Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil |
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