Detection of Lawsonia intracellularis fecal shedding in dogs in Minas Gerais, Brazil

Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin...

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Published in:Ciência rural Vol. 45; no. 9; pp. 1619 - 1621
Main Authors: Silva, Rodrigo Otávio Silveira, Gabardo, Michelle de Paula, Oliveira, Juliana Saes Vilaça de, Lobato, Francisco Carlos Faria, Guedes, Roberto Maurício Carvalho
Format: Journal Article
Language:English
Published: Universidade Federal de Santa Maria 01-09-2015
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Summary:Lawsonia intracellularisis an obligate intracellular bacterium that is responsible for proliferative enteropathy, an enteric disease endemic in swine and common in foals. However, few studies have investigated this disease in dogs, and there are no reports of dogs infected with L. intracellularisin Latin America. The aim of this study was to evaluate the fecal shedding of L. intracellularisin diarrheic and non-diarrheic dogs in Minas Gerais, Brazil. A total of 58 dogs, 18 apparently healthy and 40 diarrheic, were examined in this study. DNA extracted from feces was analyzed using a nested PCR reaction to detect L. intracellularis.Three out of 40 (7.5%) diarrheic samples, all from 3-month-old puppies, were positive for L. intracellularis. These results highlight the need for additional studies to examine the role of this pathogen as a possible cause of enteric disease in dogs Lawsonia intracellularisé uma bactéria intracelular obrigatória responsável pela enteropatia proliferativa, uma doença entérica endêmica em suínos e comum em potros. Em cães, no entanto, existem poucos estudos sobre essa doença e inexistem relatos de cães infectados por L. intracellularisna América Latina. O objetivo deste estudo foi avaliar a presença de L. intracellularisem amostras de fezes de cães diarréicos e aparentemente saudáveis em Minas Gerais, Brasil. Foram incluídos 58 cães, sendo 18 aparentemente saudáveis e 40 diarréicos. Submeteu-se o DNA bacteriano fecal a uma reação de PCR nested para L. intracellularis. Três das 40 (7,5%) amostras de cães diarréicos foram positivas para L. intracellularis, estando todos esses animais com aproximadamente 3 meses de idade. Estes resultados salientam a necessidade de mais estudos para confirmar o papel deste patógeno como uma possível causa de doença entérica em cães
ISSN:1678-4596
1678-4596
DOI:10.1590/0103-8478cr20141533