Development of complex enzymatic preparations of pactinases and celulases for sugar beet marc digestion

• Published in: Applied biochemistry and microbiology Vol. 48; no. 5; pp. 493 - 499
• Main Authors: Bushina, E. V., Rozhkova, A. M., Zorov, I. N., Satrutdinov, A. D., Bekkarevich, A. O., Koshelev, A. V., Okunev, O. N., Sinitsyn, A. P.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01-09-2012; Springer Nature B.V
• Subjects: Biochemistry; Biomedical and Life Sciences; Cellulase; Enzymes; Fermentation; Fungi; Life Sciences; Medical Microbiology; Microbiology; Sugar; Waste materials; Enzymatic Preparation; Pectin; Xylanase Activity; Apply Biochemistry; Reducer Sugar
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1134/S0003683812050031

Complex enzymatic preparations demonstrating activities homologous to pectinlyase A and heterologous to endo-1,4-β-glucanase from
Penicilliumverruculosum
and β-glycosidase from
Aspergillusniger
have been obtained on the basis of recombinant strains of the fungus
Penicilliumcanescens
. Two approaches were utilized: development of an enzymatic preparation on the basis of a new strain, which produced all three enzymes, and development of an enzymatic preparation via combined cultivation of three strains, each of which produced one of the enzymes.