Fluorescence and absorption studies of DNA–Pd(II) complex interaction: Synthesis, spectroanalytical investigations and biological activities

• Published in: Luminescence (Chichester, England) Vol. 34; no. 1; pp. 113 - 124
• Main Authors: Thakor, Khyati P., Lunagariya, Miral V., Bhatt, Bhupesh S., Patel, Mohan N.
• Format: Journal Article
• Language: English
• Published: England Wiley Subscription Services, Inc 01-02-2019
• Subjects: Absorption; absorption titration; Analytical methods; Antibacterial activity; Aquatic crustaceans; artificial metallonuclease; Bacteria; Binding sites; Conductance; Coordination compounds; Cytotoxicity; Data processing; Deoxyribonucleic acid; DNA; Electrophoretic mobility; Energy dispersive X ray spectroscopy; Fluorescence; fluorescence quenching; Free energy; Infrared radiation; Marine fishes; Mathematical analysis; Measurement; Metal complexes; Metals; Molecular docking; NMR; Nuclear magnetic resonance; Palladium; Pathogenic bacteria; Quinoline; Resistance; Saline water; Spectroscopy; Spermatozoa; Stern–Volmer equation; thermodynamic parameters; Thermogravimetric analysis; Titration; Toxicity; Viscosity; Viscosity measurement; artificial metallonuclease; absorption titration; thermodynamic parameters; cytotoxicity; Stern-Volmer equation; fluorescence quenching
• ISSN: 1522-7235; 1522-7243
• DOI: 10.1002/bio.3587

Novel palladium(II) complexes (7a–7e) of substituted quinoline derivatives were synthesized. The complexes were characterized using various techniques such as thermogravimetric analysis (TGA), elemental analysis, conductance measurement, mass, absorption, infra‐red (IR), 1H NMR, 13C NMR and energy‐dispersive X‐ray spectroscopy (EDX). Complexes for herring sperm DNA (HS DNA) binding were explored and absorption titration and the binding constant (Kb) as well as Gibb's free energy were evaluated. Complex 7d exhibited the highest binding constant, therefore the thermodynamic parameters of 7d at different temperatures were evaluated. To support the results of the absorption titration, fluorescence titration, viscosity measurement and molecular docking studies were performed. The fluorescence quenching data as evaluated from Stern–Volmer equation were used to calculate KSV, Kf and the number of binding sites. The results of all these studies were in good agreement with the absorption study. DNA electrophoretic mobility was performed to explore the possible application of metal complexes as artificial metallonucleases. The antibacterial activity of the complexes was accessed against different pathogenic bacteria and cytotoxicity was measured using brine shrimp and S. pombe.