Evaluation of the antimicrobial activity of silver nanoparticles biosynthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves

• Published in: Biotechnology and applied biochemistry Vol. 70; no. 3; pp. 1001 - 1014
• Main Authors: Ferreira, Milene Dias, Neta, Lourdes Cardoso de Souza, Brandão, Geovani Cardoso, dos Santos, Walter Nei Lopes
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01-06-2023
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; Bacteria; Electron microscopy; Factorial design; Fourier transforms; green synthesis; Herbal medicine; Infrared spectroscopy; Leaves; Light scattering; Medicinal plants; Microbicides; Microscopy; Nanoparticles; Photon correlation spectroscopy; Scanning electron microscopy; Schinus terebinthifolius; Schinus terebinthifolius Raddi; Silver; silver nanoparticles; Spectrum analysis; Synthesis; Transmission electron microscopy; antimicrobial activity; silver nanoparticles; Schinus terebinthifolius Raddi; green synthesis
• ISSN: 0885-4513; 1470-8744
• DOI: 10.1002/bab.2415

Silver nanoparticles (AgNPs) synthesized from green synthesis using medicinal plants are presented as an option for the development of new antimicrobial agents. In this context, this study aims to evaluate the antimicrobial activity of silver nanoparticles synthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves, popularly known as “aroeira.” A 23 factorial design was used to assess the statistical significance of the studied factors, and Box–Behnken design was used to define the optimal conditions for the green synthesis of AgNPs. The studied factors were significant for the synthesis of nanoparticles, and the optimal conditions were pH 9.5, reaction time of 180 min, and 10 g of S. terebinthifolius Raddi leaves. The samples were characterized using UV–Vis spectroscopy, Fourier transform infrared spectroscopy, X‐ray diffraction, scanning electron microscopy, transmission electron microscopy, and dynamic light scattering. Antimicrobial activity was evaluated using the broth microdilution method and through the minimum microbicidal concentration. AgNPs showed greater antimicrobial efficacy compared to the extract, both for bacteria and fungi: bacteriostatic activity against all tested bacteria and bactericidal against Pseudomonas aeruginosa, besides fungistatic action against Candida albicans and Candida glabrata. Finally, the aqueous extract of “aroeira” leaves was effective in the synthesis of AgNPs, with greater antimicrobial potential than the extract.
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This study aims to evaluate the antimicrobial activity of silver nanoparticles synthesized from the aqueous extract of Schinus terebinthifolius Raddi leaves.