Real‐time ALT and LDH activities determined in viable precision‐cut mouse liver slices using hyperpolarized [1‐13C]pyruvate—Implications for studies on biopsied liver tissues

Precision‐cut liver slices (PCLS) are widely used in liver research as they provide a liver model with all liver cell types in their natural architecture. The purpose of this study was to demonstrate the use of PCLS for hyperpolarized metabolic investigation in a mouse model, for potential future ap...

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Bibliographic Details
Published in:	NMR in biomedicine Vol. 32; no. 2; pp. e4043 - n/a
Main Authors:	Lev‐Cohain, Naama, Sapir, Gal, Harris, Talia, Azar, Assad, Gamliel, Ayelet, Nardi‐Schreiber, Atara, Uppala, Sivaranjan, Sosna, Jacob, Gomori, J. Moshe, Katz‐Brull, Rachel
Format:	Journal Article
Language:	English
Published:	England Wiley Subscription Services, Inc 01-02-2019
Subjects:	31P and 13C‐NMR Adenosine triphosphate Alanine Alanine transaminase Alanine Transaminase - metabolism Animals Architecture ATP Biological products Biopsy Carbon 13 Carbon Isotopes - metabolism dissolution dynamic nuclear polarization Excitation Hepatocytes L-Lactate dehydrogenase L-Lactate Dehydrogenase - metabolism lactate Lactate dehydrogenase Lactic acid Liver Liver - enzymology Liver - pathology Male Metabolism Metabolites Metabolome Mice, Inbred ICR NMR Nuclear magnetic resonance perfusion pyruvate Pyruvic acid Pyruvic Acid - metabolism Spectra Tissues Viability dissolution dynamic nuclear polarization perfusion liver 31P and 13C-NMR alanine metabolism lactate pyruvate
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