Study of wound healing in rats treated with topical and injected mitomycin-C

• Published in: Brazilian journal of otorhinolaryngology Vol. 74; no. 3; pp. 328 - 330
• Main Authors: de Andrade Quintanilha Ribeiro, Fernando, de Pádua Borges, Janaína, Guaraldo, Lusiele, Vianna, Maria Regina
• Format: Journal Article
• Language: English
• Published: Brazil Elsevier Editora Ltda 01-05-2008; Elsevier
• Subjects: Administration, Topical; Alkylating Agents - administration & dosage; Animals; Fibroblasts - drug effects; Fibrosis - prevention & control; Male; Mitomycin - administration & dosage; mitomycin-C; Original; Rats; Rats, Wistar; Time Factors; topical; wound healing; Wound Healing - drug effects; mitomycin-C; wound healing; topical; rats
• ISSN: 1808-8694; 1808-8686
• DOI: 10.1016/S1808-8694(15)30563-2

Experimental study in animals. Introduction: Mitomycin C has been used as a fibroblasts inhibitor, thus reducing the scaring process in surgical wounds.
This paper aims at assessing the use of Mitomycin C to reduce the scaring process through its topical use and later injected reinforcements.
We used a model of a creating a wound in the dorsum of rats, removing a circular piece of skin and letting it heal by itself. We used 18 rats, divided in three groups. The first group - Control, the second with topical use, and a third group with injected mitomycin C reinforcement, monthly for 2 months. After 3 months the animals were slaughtered and the scars were surgically removed and sent for histology study.
We noticed, under different criteria, that healing with topical mitomycin is less intense; however, when it was injected, the parameters were again comparable to those from the control group.
We believe that injected mitomycin C in the scar, since it is highly toxic, it destroys tissue and brings about scar neoformation.
Mitomycin C reduces the scaring process when used topically; however, it increases scar tissue formation when injected in these wounds.