First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

• Published in: Journal of Zhejiang University. B. Science Vol. 8; no. 5; pp. 345 - 351
• Main Authors: Jiang, Li-qin, Li, Xu-qing, Zheng, Jing-wu
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01-05-2007; Institute of Biotechnology, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; Zhejiang University Press
• Subjects: Animals; Biological Evolution; Biotechnology; China; DNA, Helminth - genetics; Genetics; Nematoda - anatomy & histology; Nematoda - genetics; Phylogeny; Pinus - parasitology; Species Specificity; 伞滑刃线虫; 华东地区; 新记录; 系统关系; China; Bursaphelenchus rainulfi; Pine wood; Phylogeny; Morphometrics; Morphology; ITS-RFLP
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.2007.B0345

Bursaphelenchus rainulfi isolated from dead pine trees in Zbejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit （LSU） rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.