Comparative studies on lectin-carbohydrate interactions in low and high density homo- and heteroglycoclusters

• Published in: Organic & biomolecular chemistry Vol. 8; no. 8; p. 1849
• Main Authors: Gómez-García, Marta, Benito, Juan M, Gutiérrez-Gallego, Ricardo, Maestre, Alfredo, Mellet, Carmen Ortiz, Fernández, José M García, Blanco, José L Jiménez
• Format: Journal Article
• Language: English
• Published: England 01-01-2010
• Subjects: Binding Sites; Carbohydrate Metabolism; Carbohydrate Sequence; Carbohydrates - chemical synthesis; Carbohydrates - chemistry; Concanavalin A - metabolism; Enzyme Assays; Glycoconjugates - chemical synthesis; Glycoconjugates - chemistry; Glycoconjugates - metabolism; Lectins - metabolism; Models, Molecular; Molecular Sequence Data; Surface Plasmon Resonance
• ISSN: 1477-0539
• DOI: 10.1039/b920048g

A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of "non-active" structural motifs in the presence of a certain density of "active" glycoligands.