In vitro plant regeneration of Ilex paraguariensis (Aquifoliaceae)

• Published in: In vitro cellular & developmental biology. Plant Vol. 35; no. 5; pp. 401 - 402
• Main Authors: Sansberro, P, Rey, H, Mroginski, L, Collavino, M
• Format: Journal Article
• Language: English
• Published: Wallingford Society for In Vitro Biology 01-09-1999; Cambridge University Press
• Subjects: Agroforestry; Apical meristems; Aquifoliaceae; Biological and medical sciences; Biotechnology; cell differentiation; culture media; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Ilex paraguariensis; In vitro propagation: entire plant regeneration from tissues and cell cultures; indole butyric acid; indole-3-butyric acid; Mate; Methods. Procedures. Technologies; Micropropagation; Plant cells and fungal cells; Plant growth regulators; Plants; Protocols and Research Reports; regenerative ability; roots; Shoot tip culture; shoots; sucrose; tissue culture; Aquifoliaceae; Culture medium; Regeneration; Auxin; Sucrose; Dicotyledones; Angiospermae; Stimulant plant; Plant growth substance; Spermatophyta; Chemical composition; Micropropagation
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-999-0054-5

Nodal segments as well as shoot tips and apical meristems of 2-yr-old "maté" plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) medium with 3% sucrose. We induced roots to differentiate by transferring the regenerated shoots onto the same medium, solidified with 2.5 g Phytagel per l and supplemented with indole-3-butyric acid (7.4 µM) and finally transferring shoots to 1/4 MS medium with 3% sucrose and lacking growth regulators. Plants were successfully established in soil.