Dual function of the transcription factor Ftz-f1 on oviposition in the cockroach Blattella germanica

• Published in: Insect molecular biology Vol. 32; no. 6; pp. 689 - 702
• Main Authors: Alborzi, Zeynab, Piulachs, Maria-Dolors
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01-12-2023
• Subjects: Blattella germanica; Cockroaches; Cytoskeleton; Females; Follicles; Gene expression; Genes; Insects; Molting; Oogenesis; Ovaries; Oviposition; Ovulation; Pituitary (anterior); Transcription factors; panoistic ovary; F-actin; cytoskeleton; ecdysone; cockroach
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/imb.12866

The transcription factor Ftz-f1 has multiple functions in insect development in a spatial-temporal line. One of these roles is in the insect ovaries, specifically in the regulation of steroidogenic enzymes production. We studied the function of F in Blattella germanica oogenesis, as it shows two moments of high expression in ovaries: before the imaginal moult, and just before ovulation in the adult. Injecting dsftz-f1 into adult females, either just after the imaginal moult or just prior to choriogenesis, prevented oviposition, with differences between the two approaches. In 3-day-old adult females treated with dsftz-f1 just after the emergence, the expression of ftz-f1 was not modified, but the steroidogenic genes increased their expression. ftz-f1 transcript levels in the ovaries of 5-day-old dsftz-f1-treated females were significantly depleted, and the expression levels of the same steroidogenic genes began to decrease. These results suggest that Ftz-f1 regulates the expression of steroidogenic genes in B. germanica, with phm possibly being a key target. Ftz-f1 has a different temporal function in the cytoskeleton of follicular cells of the basal ovarian follicles. Early in the gonadotrophic cycle, Ftz-f1 promotes the expression of genes related to the cytoskeleton and muscle proteins, while at the end of the cycle it maintains the expression levels of these genes, thus ensuring correct ovulation.