Inhibition of multicycle influenza virus replication by hybrid antibody- directed cytotoxic T lymphocyte lysis

• Published in: The Journal of immunology (1950) Vol. 146; no. 1; pp. 321 - 326
• Main Authors: Moran, TM, Usuba, O, Kuzu, H, Kuzu, Y, Schulman, J, Bona, CA
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01-01-1991; American Association of Immunologists
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Biological and medical sciences; Cells, Cultured; Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others); Cytotoxicity, Immunologic; Dogs; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Hybridomas; Immunity, Cellular; Immunobiology; Immunological reactions in vitro; In Vitro Techniques; Influenza A virus - growth & development; Influenza A virus - immunology; Receptors, Antigen, T-Cell - immunology; Receptors, Antigen, T-Cell, alpha-beta; T-Lymphocytes, Cytotoxic - immunology; Virus Replication; Virus; Antibody; T-Lymphocyte; Molecular hybrid; Hybridoma; Orthomyxoviridae; Cytotoxicity; Replication; Target cell; Inhibition; Mechanism of action; Influenzavirus
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.146.1.321

Bifunctional antibodies with specificity for the TCR/CD3 complex as well as to a target cell-surface Ag can redirect CTL to lyse the target cell. We have produced a hybrid hybridoma, HHA6, which secretes bifunctional antibodies capable of redirecting CTL to lyse influenza virus-infected target cells. When added along with CTL to virus-infected cells, these antibodies very efficiently inhibit multicycle virus replication. Because hybrid hybridomas reassort H and L chains randomly we attempted to purify bifunctional antibody by using HPLC. The purification increased the potency of HHA6. This increase probably reflects an enrichment of the active species and the removal of inhibiting antibody species.