Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay: a preliminary step toward treating sensorineural hearing loss
Purpose Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In...
Saved in:
| Published in: | European archives of oto-rhino-laryngology Vol. 279; no. 4; pp. 1805 - 1812 |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Berlin/Heidelberg
Springer Berlin Heidelberg
01-04-2022
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Purpose
Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro.
Methods
DPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell.
Results
DPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment.
Conclusion
We found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL |
|---|---|
| AbstractList | Purpose
Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro.
Methods
DPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell.
Results
DPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment.
Conclusion
We found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro. DPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell. DPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment. We found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL. PURPOSESensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity. However, regenerative medicine, such as stem cell therapy, has become a promising tool to cure many diseases, including hearing loss. In this study, we determined whether DPSCs could differentiate into cochlear hair cell in vitro. METHODSDPSCs derived from human third molar dental pulp were induced into NSCs using a medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) for 7 days, and then into cochlear hair cell using a medium containing EGF and IGF-1 for the next 14 days. We used the neuroepithelial protein marker nestin and cochlear hair cell marker myosin VIIa as the markers for cells differentiation. Cells expressing the positive markers under the microscope were confirmed to have differentiated into cochlear hair cell. RESULTSDPSCs were successfully induced to differentiate into NSCs, with mean 24% nestin-positive cells. We found that DPSC-derived NSCs have a great capacity in differentiating into inner ear hair cell-like cells with an average of 81% cells presenting myosin VIIa. Thus, DPSCs have high potential to serve as a good resource for SNHL treatment. CONCLUSIONWe found the high potential of DPSCs to differentiate into NSC. The ability of DPSCs in differentiating into neural lineage cell made them a good candidate for regenerative therapy in neural diseases, such as SNHL. |
| Author | Sandra, Ferry Bashiruddin, Jenny Adriztina, Indri Putra, Imam Budi Ichwan, Muhamad Sartika, Cynthia Retna Munir, Delfitri Pratiwi, Endah Dianty Farhat Sembiring, Rosita Juwita Chouw, Angliana |
| Author_xml | – sequence: 1 givenname: Indri orcidid: 0000-0003-0651-9594 surname: Adriztina fullname: Adriztina, Indri email: adriztina@yahoo.com, indri.adriztina@usu.ac.id organization: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara – sequence: 2 givenname: Delfitri surname: Munir fullname: Munir, Delfitri organization: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara – sequence: 3 givenname: Ferry surname: Sandra fullname: Sandra, Ferry organization: Division of Oral Biology, Departement of Biochemistry and Molecular Biology, Faculty of Dentistry, Trisakti University – sequence: 4 givenname: Muhamad surname: Ichwan fullname: Ichwan, Muhamad organization: Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Sumatera Utara – sequence: 5 givenname: Jenny surname: Bashiruddin fullname: Bashiruddin, Jenny organization: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, University of Indonesia – sequence: 6 givenname: Imam Budi surname: Putra fullname: Putra, Imam Budi organization: Department of Dermatovenerology, Faculty of Medicine, Universitas Sumatera Utara – sequence: 7 surname: Farhat fullname: Farhat organization: Department of Otorhinolaryngology-Head and Neck Surgery, Faculty of Medicine, Universitas Sumatera Utara – sequence: 8 givenname: Rosita Juwita surname: Sembiring fullname: Sembiring, Rosita Juwita organization: Department of Clinical Pathology, Faculty of Medicine, Universitas Sumatera Utara – sequence: 9 givenname: Cynthia Retna surname: Sartika fullname: Sartika, Cynthia Retna organization: Prodia Stemcell Indonesia – sequence: 10 givenname: Angliana surname: Chouw fullname: Chouw, Angliana organization: Prodia Stemcell Indonesia – sequence: 11 givenname: Endah Dianty surname: Pratiwi fullname: Pratiwi, Endah Dianty organization: Prodia Stemcell Indonesia |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/34008035$$D View this record in MEDLINE/PubMed |
| BookMark | eNp9Uc1u1jAQtFAR_Vp4AQ7IRy4p_k1ibqiFglSJC5yt_ZI1dZXYwXZA3-PwpjikcOSyK-3OzO5oLshZiAEJecnZFWese5MZU0w3TPCGtX2rGvOEHLiSqlGdaM_IgRnZNUp13Tm5yPmBMaaVkc_IuVSM9UzqA_l1453DhKF4KD4GOsACgy8nGh0d6xgmuqzTQnPBmQ44TdSHEmsJmChCovfg075Ysw_fKIS6pD98SZFCznB6S4EuCSc_-wDptCkttMSfkEZaEtazlZUx5Jh8wDXVi_dVeJtOMefn5KmDKeOLx35Jvn54_-X6Y3P3-fbT9bu7ZpCqK40TbcvRaSd6JbWWTHPXDwZMh9oMvVGgjwMI6bRG3h3l6IzmxxGcU6I1TshL8nrXXVL8vmIudvZ58wUB45qt0KI3QvVSVajYoUOqDyZ0dkl-rt4sZ3aLxu7R2BqN_RONNZX06lF_Pc44_qP8zaIC5A7Iy2Yek32IawrV8_9kfwPybZ8D |
| CitedBy_id | crossref_primary_10_3390_cells11182792 crossref_primary_10_3390_cells12131686 |
| Cites_doi | 10.1590/S0103-64402011000200001 10.2217/rme.13.32 10.1016/j.heares.2007.06.007 10.4252/wjsc.v7.i5.839 10.3109/00016489.2011.603135 10.1159/000486772 10.3727/000000006783981819 10.1016/j.pneurobio.2011.01.004 10.1007/s00418-011-0858-3 10.1007/s10162-005-0012-9 10.1080/14653240600855905 10.1007/s00018-018-2794-z 10.1073/pnas.2334503100 10.1634/stemcells.2006-0390 10.1016/j.drudis.2010.02.006 10.1016/j.archoralbio.2012.08.010 10.1016/j.jfma.2014.09.003 10.1073/pnas.240309797 10.3390/jfb2030249 10.1634/stemcells.2007-0979 10.1038/nprot.2006.33 10.7874/kja.2012.16.2.47 10.1517/14712598.6.9.847 10.1080/000164801317166880 10.1016/S1672-2930(07)50020-6 |
| ContentType | Journal Article |
| Copyright | The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
| Copyright_xml | – notice: The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 – notice: 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
| DBID | NPM AAYXX CITATION 7X8 |
| DOI | 10.1007/s00405-021-06864-9 |
| DatabaseName | PubMed CrossRef MEDLINE - Academic |
| DatabaseTitle | PubMed CrossRef MEDLINE - Academic |
| DatabaseTitleList | PubMed MEDLINE - Academic |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1434-4726 |
| EndPage | 1812 |
| ExternalDocumentID | 10_1007_s00405_021_06864_9 34008035 |
| Genre | Journal Article |
| GrantInformation_xml | – fundername: The Prodia Education and Research Institute |
| GroupedDBID | --- -53 -5E -5G -BR -EM -Y2 -~C .55 .86 .GJ .VR 06C 06D 0R~ 0VY 1N0 1SB 203 28- 29G 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 36B 4.4 406 408 409 40D 40E 53G 5QI 5VS 67Z 6NX 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AABYN AAFGU AAHNG AAIAL AAJKR AAKSU AANXM AANZL AAPBV AARHV AARTL AATNV AATVU AAUYE AAWCG AAYFA AAYIU AAYQN AAYTO ABBBX ABBXA ABDZT ABECU ABFGW ABFTV ABHLI ABHQN ABIPD ABJNI ABJOX ABKAS ABKCH ABKTR ABLJU ABMNI ABMQK ABNWP ABPLI ABPTK ABQBU ABSXP ABTEG ABTKH ABTMW ABULA ABWNU ABXPI ACBMV ACBRV ACBXY ACBYP ACGFS ACHSB ACHXU ACIGE ACIPQ ACKNC ACMDZ ACMLO ACOKC ACOMO ACTTH ACUDM ACVWB ACWMK ADHHG ADHIR ADINQ ADJJI ADKNI ADKPE ADMDM ADOXG ADRFC ADTPH ADURQ ADYFF ADZKW AEBTG AEEQQ AEFIE AEFTE AEGAL AEGNC AEJHL AEJRE AEKMD AENEX AEOHA AEPYU AESKC AESTI AETLH AEVLU AEVTX AEXYK AFAFS AFEXP AFLOW AFNRJ AFQWF AFWTZ AFZKB AGAYW AGDGC AGGBP AGGDS AGJBK AGKHE AGMZJ AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHIZS AHKAY AHSBF AHYZX AIAKS AIIXL AILAN AIMYW AITGF AJBLW AJDOV AJRNO AJZVZ AKMHD AKQUC ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARMRJ AXYYD AZFZN B-. BA0 BBWZM BDATZ BGNMA CAG COF CSCUP DDRTE DL5 DNIVK DPUIP DU5 EBD EBLON EBS EIOEI EJD EMB EMOBN EN4 ESBYG F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GRRUI GXS HF~ HG5 HG6 HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ IMOTQ IWAJR IXC IXD IXE IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH LAS LLZTM M4Y MA- N2Q N9A NB0 NDZJH NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM P19 P2P P9S PF0 PT4 PT5 QOK QOR QOS R89 R9I RHV RIG RNI ROL RPX RRX RSV RZK S16 S1Z S26 S27 S28 S37 S3B SAP SCLPG SDE SDH SDM SHX SISQX SJYHP SMD SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZ9 SZN T13 T16 TSG TSK TSV TT1 TUC U2A U9L UG4 UNUBA UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WJK WK8 X7M YLTOR Z45 Z7U Z7V Z7X Z81 Z82 Z83 Z87 Z8O Z8P Z8U Z8V Z8W Z91 ZMTXR ZOVNA ~A9 ~EX AACDK AAEOY AAJBT AASML AAYZH ABAKF ACAOD ACDTI ACZOJ AEFQL AEMSY AFBBN AGQEE AGRTI AIGIU AJOOF H13 NPM AAYXX CITATION 7X8 |
| ID | FETCH-LOGICAL-c347t-f2661ef5f2843553051f8c9a97e59c894a5bca23f55e17b3df951bdaff4269f23 |
| IEDL.DBID | AEJHL |
| ISSN | 0937-4477 |
| IngestDate | Fri Oct 25 06:21:01 EDT 2024 Thu Nov 21 23:28:45 EST 2024 Wed Oct 16 00:42:47 EDT 2024 Sat Dec 16 12:07:47 EST 2023 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | Dental pulp stem cells Sensorineural hearing loss Cochlear hair cell |
| Language | English |
| License | 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c347t-f2661ef5f2843553051f8c9a97e59c894a5bca23f55e17b3df951bdaff4269f23 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ORCID | 0000-0003-0651-9594 |
| PMID | 34008035 |
| PQID | 2528924834 |
| PQPubID | 23479 |
| PageCount | 8 |
| ParticipantIDs | proquest_miscellaneous_2528924834 crossref_primary_10_1007_s00405_021_06864_9 pubmed_primary_34008035 springer_journals_10_1007_s00405_021_06864_9 |
| PublicationCentury | 2000 |
| PublicationDate | 2022-04-01 |
| PublicationDateYYYYMMDD | 2022-04-01 |
| PublicationDate_xml | – month: 04 year: 2022 text: 2022-04-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | Berlin/Heidelberg |
| PublicationPlace_xml | – name: Berlin/Heidelberg – name: Germany |
| PublicationSubtitle | and Head & Neck |
| PublicationTitle | European archives of oto-rhino-laryngology |
| PublicationTitleAbbrev | Eur Arch Otorhinolaryngol |
| PublicationTitleAlternate | Eur Arch Otorhinolaryngol |
| PublicationYear | 2022 |
| Publisher | Springer Berlin Heidelberg |
| Publisher_xml | – name: Springer Berlin Heidelberg |
| References | Li, Roblin, Liu, Heller (CR8) 2003; 100 Chang, Chang, Tsai, Chang, Lin (CR10) 2014; 113 Bernal, Arranz (CR18) 2018; 75 Ito, Kojima, Kawaguchi (CR19) 2001; 121 Arthur, Rychkov, Shi, Koblar, Gronthos (CR15) 2008; 26 Ying, Min-min, Hai-bo, Sonh-tao (CR7) 2007; 2 Potdar, Jethmalani (CR24) 2015; 7 Pacey, Stead, Gleave, Tomezyk, Doering (CR22) 2006; 1 Lee, Kang, Seo, Choi, Lee, Kim, Park (CR20) 2012; 16 Doyle, Kazda, Hort, Mckay, Oleskevich (CR9) 2007; 25 Parker, Corliss, Gray, Anderson, Bobbin, Snyder, Cotanche (CR4) 2007; 232 Qin, Zhao, Sun, Ren, Gou, Liu (CR6) 2011; 131 Hildebrand, Dahl, Hardman, Coleman, Shephard, de Silva (CR27) 2005; 6 Hu, Ulfendahl (CR2) 2004; 8 Karaoz, Demircan, Saglam, Aksoy, Kaymaz, Duruksu (CR14) 2011; 136 Zhang, Zhou, Li, Wang, Yang, Li, Cao, Fu (CR16) 2018; 48 Gronthos, Mankani, Brahim, Robey, Shi (CR13) 2000; 97 Estrela, Alencar, Kitten, Vencio, Gava (CR26) 2011; 22 Coleman, Hardman, Coco, Epp, de Silva, Crook (CR3) 2006; 15 Kawashima (CR11) 2012; 57 Mahmoidain-sani, Hashemzadeh-chalashtori, Jami, Saidijam (CR21) 2017; 21 Rivolta (CR1) 2010; 15 Devarajan, Staecker, Detamore (CR23) 2011; 2 Dominici, Blanc, Mueller, Sleper-Conterbach, Marini, Krause (CR12) 2006; 8 Alsberg, Recum, Mahoney (CR17) 2006; 6 Appler, Goodrich (CR25) 2011; 93 Cho, Cho, Jang, Jeong, Park (CR5) 2011; 26 KL Doyle (6864_CR9) 2007; 25 A Bernal (6864_CR18) 2018; 75 B Coleman (6864_CR3) 2006; 15 E Alsberg (6864_CR17) 2006; 6 E Karaoz (6864_CR14) 2011; 136 YB Cho (6864_CR5) 2011; 26 MR Mahmoidain-sani (6864_CR21) 2017; 21 PD Potdar (6864_CR24) 2015; 7 X Zhang (6864_CR16) 2018; 48 CC Chang (6864_CR10) 2014; 113 S Gronthos (6864_CR13) 2000; 97 JH Lee (6864_CR20) 2012; 16 W Ying (6864_CR7) 2007; 2 A Arthur (6864_CR15) 2008; 26 N Kawashima (6864_CR11) 2012; 57 LKK Pacey (6864_CR22) 2006; 1 K Devarajan (6864_CR23) 2011; 2 Z Hu (6864_CR2) 2004; 8 MS Hildebrand (6864_CR27) 2005; 6 H Li (6864_CR8) 2003; 100 C Estrela (6864_CR26) 2011; 22 J Ito (6864_CR19) 2001; 121 M Dominici (6864_CR12) 2006; 8 JM Appler (6864_CR25) 2011; 93 MN Rivolta (6864_CR1) 2010; 15 MA Parker (6864_CR4) 2007; 232 H Qin (6864_CR6) 2011; 131 |
| References_xml | – volume: 22 start-page: 91 year: 2011 end-page: 98 ident: CR26 article-title: Mesenchymal stem cells in the dental tissue: perspectives for tissue regeneration publication-title: Braz Dent J doi: 10.1590/S0103-64402011000200001 contributor: fullname: Gava – volume: 8 start-page: 309 issue: 3 year: 2004 end-page: 318 ident: CR2 article-title: The potential of stem cells for the restoration of auditory function in humans publication-title: Regen Med doi: 10.2217/rme.13.32 contributor: fullname: Ulfendahl – volume: 26 start-page: 492 year: 2011 end-page: 498 ident: CR5 article-title: Transplantation of neural differentiated human mesenchymal stem cells into the cochlea of an auditory-neuropathy guinea pig model publication-title: Cell Ther Organ Transplant contributor: fullname: Park – volume: 232 start-page: 29 issue: 1–2 year: 2007 end-page: 43 ident: CR4 article-title: Neural stem cells injected into the sound-damaged cochlea migrate throughout the cochlea and express markers of hair cells, supporting cells, and spiral ganglion cells publication-title: Hear Res doi: 10.1016/j.heares.2007.06.007 contributor: fullname: Cotanche – volume: 7 start-page: 839 issue: 5 year: 2015 end-page: 851 ident: CR24 article-title: Human dental pulp stem cells: applications in future regenerative medicine publication-title: World J Stem cells doi: 10.4252/wjsc.v7.i5.839 contributor: fullname: Jethmalani – volume: 131 start-page: 1136 year: 2011 end-page: 1141 ident: CR6 article-title: The differentiation of mesenchymal stem cells into inner ear hair cell-like cells in vitro publication-title: Acta Otolaryngol doi: 10.3109/00016489.2011.603135 contributor: fullname: Liu – volume: 48 start-page: 258 year: 2018 end-page: 266 ident: CR16 article-title: Transplanted dental pulp stem cell migrate to injured area and express neural markers in a rat model cerebral ischemia publication-title: Cell Physiol Biochem doi: 10.1159/000486772 contributor: fullname: Fu – volume: 15 start-page: 369 year: 2006 end-page: 380 ident: CR3 article-title: Fate of embryonic stem cells transplanted into the deafened mammalian cochlea publication-title: Cell Transpl doi: 10.3727/000000006783981819 contributor: fullname: Crook – volume: 93 start-page: 488 issue: 4 year: 2011 end-page: 550 ident: CR25 article-title: Connecting the ear to the brain: molecular mechanism of auditory circuit assembly publication-title: Prog Neurobiol doi: 10.1016/j.pneurobio.2011.01.004 contributor: fullname: Goodrich – volume: 136 start-page: 455 year: 2011 end-page: 473 ident: CR14 article-title: Human dental pulp demonstrate better neural and epithelial stem cell properties than bone marrow-derived mesenchymal stem cells publication-title: Histochem Cell Biol doi: 10.1007/s00418-011-0858-3 contributor: fullname: Duruksu – volume: 6 start-page: 341 year: 2005 end-page: 345 ident: CR27 article-title: Survival of partially differentiated mouse embryonic stem cells in the scala media of the guinea pig cochlea publication-title: J Assoc Res Otolaryngol doi: 10.1007/s10162-005-0012-9 contributor: fullname: de Silva – volume: 8 start-page: 315 issue: 4 year: 2006 end-page: 317 ident: CR12 article-title: Minimal criteria for defining multipotent mesenchymal stromal cell. The international society for cellular therapy position statement publication-title: Cytotherapy doi: 10.1080/14653240600855905 contributor: fullname: Krause – volume: 75 start-page: 2177 year: 2018 end-page: 2194 ident: CR18 article-title: Nestin-expressing progenitor cells: function, identity and therapeutic implications publication-title: Cel Mol Life Sci doi: 10.1007/s00018-018-2794-z contributor: fullname: Arranz – volume: 100 start-page: 13495 year: 2003 end-page: 13500 ident: CR8 article-title: Generation of hair cells by stepwise differentiation of embryonic stem cells publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.2334503100 contributor: fullname: Heller – volume: 25 start-page: 621 year: 2007 end-page: 627 ident: CR9 article-title: Differentiation of adult mouse olfactory precursor cells into hair cell in vitro publication-title: Stem Cells doi: 10.1634/stemcells.2006-0390 contributor: fullname: Oleskevich – volume: 15 start-page: 283 issue: 7/8 year: 2010 end-page: 286 ident: CR1 article-title: Stem cells and cell lines from the human auditory organ: applications, hurdles and bottlenecks in the development of regenerative therapies for deafness publication-title: Drug Discov Today doi: 10.1016/j.drudis.2010.02.006 contributor: fullname: Rivolta – volume: 57 start-page: 1439 year: 2012 end-page: 1458 ident: CR11 article-title: Characterisation of dental pulp stem cells: a new horizon for tissue regeneration? publication-title: Arch Oral Biol doi: 10.1016/j.archoralbio.2012.08.010 contributor: fullname: Kawashima – volume: 113 start-page: 956 year: 2014 end-page: 965 ident: CR10 article-title: Neurogenic differentiation of dental pulp stem cells to neuron-like cells in dopaminergic and motor neuronal inductive media publication-title: J Formos Med Assoc doi: 10.1016/j.jfma.2014.09.003 contributor: fullname: Lin – volume: 97 start-page: 13625 year: 2000 end-page: 13630 ident: CR13 article-title: Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo publication-title: Proc Natl Sci USA doi: 10.1073/pnas.240309797 contributor: fullname: Shi – volume: 2 start-page: 249 year: 2011 end-page: 270 ident: CR23 article-title: A review of gene delivery and stem cell based therapies for regenerating inner ear hair cells publication-title: J Funct Biomater doi: 10.3390/jfb2030249 contributor: fullname: Detamore – volume: 26 start-page: 1787 year: 2008 end-page: 1795 ident: CR15 article-title: Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues publication-title: Stem Cells doi: 10.1634/stemcells.2007-0979 contributor: fullname: Gronthos – volume: 1 start-page: 215 year: 2006 end-page: 222 ident: CR22 article-title: Neural stem cell culture: neurosphere generation, microscopical analysis and cryopreservation publication-title: Nat Protoc doi: 10.1038/nprot.2006.33 contributor: fullname: Doering – volume: 16 start-page: 47 year: 2012 end-page: 53 ident: CR20 article-title: Neural differentiation of bone marrow-derived mesenchymal stem cells: applicability for inner ear therapy publication-title: Korean Audiol Soc doi: 10.7874/kja.2012.16.2.47 contributor: fullname: Park – volume: 6 start-page: 847 issue: 9 year: 2006 end-page: 866 ident: CR17 article-title: Environmental cues to guide stem cell fate decision for tissue engineering application publication-title: Expert Opin Biol Ther doi: 10.1517/14712598.6.9.847 contributor: fullname: Mahoney – volume: 21 start-page: 179 issue: 2 year: 2017 end-page: 184 ident: CR21 article-title: In vitro differentiation of human bone marrow mesenchymal stem cells to hair cells using growth factors publication-title: Int Tinnitus J contributor: fullname: Saidijam – volume: 121 start-page: 140 year: 2001 end-page: 142 ident: CR19 article-title: Survival of neural stem cells in the cochlea publication-title: Acta Otolaryngol doi: 10.1080/000164801317166880 contributor: fullname: Kawaguchi – volume: 2 start-page: 97 issue: 2 year: 2007 end-page: 100 ident: CR7 article-title: In vitro differentiation of adipose-derived stem cells into hair cell-like cells in guinea pigs publication-title: J Otol doi: 10.1016/S1672-2930(07)50020-6 contributor: fullname: Sonh-tao – volume: 121 start-page: 140 year: 2001 ident: 6864_CR19 publication-title: Acta Otolaryngol doi: 10.1080/000164801317166880 contributor: fullname: J Ito – volume: 2 start-page: 97 issue: 2 year: 2007 ident: 6864_CR7 publication-title: J Otol doi: 10.1016/S1672-2930(07)50020-6 contributor: fullname: W Ying – volume: 232 start-page: 29 issue: 1–2 year: 2007 ident: 6864_CR4 publication-title: Hear Res doi: 10.1016/j.heares.2007.06.007 contributor: fullname: MA Parker – volume: 2 start-page: 249 year: 2011 ident: 6864_CR23 publication-title: J Funct Biomater doi: 10.3390/jfb2030249 contributor: fullname: K Devarajan – volume: 7 start-page: 839 issue: 5 year: 2015 ident: 6864_CR24 publication-title: World J Stem cells doi: 10.4252/wjsc.v7.i5.839 contributor: fullname: PD Potdar – volume: 15 start-page: 369 year: 2006 ident: 6864_CR3 publication-title: Cell Transpl doi: 10.3727/000000006783981819 contributor: fullname: B Coleman – volume: 75 start-page: 2177 year: 2018 ident: 6864_CR18 publication-title: Cel Mol Life Sci doi: 10.1007/s00018-018-2794-z contributor: fullname: A Bernal – volume: 57 start-page: 1439 year: 2012 ident: 6864_CR11 publication-title: Arch Oral Biol doi: 10.1016/j.archoralbio.2012.08.010 contributor: fullname: N Kawashima – volume: 97 start-page: 13625 year: 2000 ident: 6864_CR13 publication-title: Proc Natl Sci USA doi: 10.1073/pnas.240309797 contributor: fullname: S Gronthos – volume: 6 start-page: 341 year: 2005 ident: 6864_CR27 publication-title: J Assoc Res Otolaryngol doi: 10.1007/s10162-005-0012-9 contributor: fullname: MS Hildebrand – volume: 22 start-page: 91 year: 2011 ident: 6864_CR26 publication-title: Braz Dent J doi: 10.1590/S0103-64402011000200001 contributor: fullname: C Estrela – volume: 25 start-page: 621 year: 2007 ident: 6864_CR9 publication-title: Stem Cells doi: 10.1634/stemcells.2006-0390 contributor: fullname: KL Doyle – volume: 136 start-page: 455 year: 2011 ident: 6864_CR14 publication-title: Histochem Cell Biol doi: 10.1007/s00418-011-0858-3 contributor: fullname: E Karaoz – volume: 48 start-page: 258 year: 2018 ident: 6864_CR16 publication-title: Cell Physiol Biochem doi: 10.1159/000486772 contributor: fullname: X Zhang – volume: 100 start-page: 13495 year: 2003 ident: 6864_CR8 publication-title: Proc Natl Acad Sci doi: 10.1073/pnas.2334503100 contributor: fullname: H Li – volume: 93 start-page: 488 issue: 4 year: 2011 ident: 6864_CR25 publication-title: Prog Neurobiol doi: 10.1016/j.pneurobio.2011.01.004 contributor: fullname: JM Appler – volume: 16 start-page: 47 year: 2012 ident: 6864_CR20 publication-title: Korean Audiol Soc doi: 10.7874/kja.2012.16.2.47 contributor: fullname: JH Lee – volume: 8 start-page: 315 issue: 4 year: 2006 ident: 6864_CR12 publication-title: Cytotherapy doi: 10.1080/14653240600855905 contributor: fullname: M Dominici – volume: 1 start-page: 215 year: 2006 ident: 6864_CR22 publication-title: Nat Protoc doi: 10.1038/nprot.2006.33 contributor: fullname: LKK Pacey – volume: 15 start-page: 283 issue: 7/8 year: 2010 ident: 6864_CR1 publication-title: Drug Discov Today doi: 10.1016/j.drudis.2010.02.006 contributor: fullname: MN Rivolta – volume: 113 start-page: 956 year: 2014 ident: 6864_CR10 publication-title: J Formos Med Assoc doi: 10.1016/j.jfma.2014.09.003 contributor: fullname: CC Chang – volume: 21 start-page: 179 issue: 2 year: 2017 ident: 6864_CR21 publication-title: Int Tinnitus J contributor: fullname: MR Mahmoidain-sani – volume: 26 start-page: 1787 year: 2008 ident: 6864_CR15 publication-title: Stem Cells doi: 10.1634/stemcells.2007-0979 contributor: fullname: A Arthur – volume: 8 start-page: 309 issue: 3 year: 2004 ident: 6864_CR2 publication-title: Regen Med doi: 10.2217/rme.13.32 contributor: fullname: Z Hu – volume: 26 start-page: 492 year: 2011 ident: 6864_CR5 publication-title: Cell Ther Organ Transplant contributor: fullname: YB Cho – volume: 131 start-page: 1136 year: 2011 ident: 6864_CR6 publication-title: Acta Otolaryngol doi: 10.3109/00016489.2011.603135 contributor: fullname: H Qin – volume: 6 start-page: 847 issue: 9 year: 2006 ident: 6864_CR17 publication-title: Expert Opin Biol Ther doi: 10.1517/14712598.6.9.847 contributor: fullname: E Alsberg |
| SSID | ssj0005493 |
| Score | 2.372214 |
| Snippet | Purpose
Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative... Sensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative capacity.... PURPOSESensorineural hearing loss (SNHL) is commonly caused by the death or dysfunction of cochlear cell types as a result of their lack of regenerative... |
| SourceID | proquest crossref pubmed springer |
| SourceType | Aggregation Database Index Database Publisher |
| StartPage | 1805 |
| SubjectTerms | Head and Neck Surgery Medicine Medicine & Public Health Neurosurgery Otology Otorhinolaryngology |
| Title | Differentiation capacity of dental pulp stem cell into inner ear hair cell using an in vitro assay: a preliminary step toward treating sensorineural hearing loss |
| URI | https://link.springer.com/article/10.1007/s00405-021-06864-9 https://www.ncbi.nlm.nih.gov/pubmed/34008035 https://search.proquest.com/docview/2528924834 |
| Volume | 279 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bi9QwFD64uyC-eL-MN47gm0Z2mmTb-LY4swyivqiw-1SSJtHBoS2dzsL-nP2nnpN2RmRF0KdCk6blnJN-X5JzAXjpjYshmKnwh8EJ5aognIyFcMYpr_w08-lEd_E5_3RazOacJkfuti7qH2-2J5LpR72LdWNz42BidtQpjpQwe3BA2KPJuA-O5-8XH355dqgh1y4hr1Aqz8dYmT-P8jseXSGZVw5IE-6c3PqvL74NN0eaiceDXdyBa6G-C9c_jgfp9-ByNhZG6QfVYEWgWREjxyaiTyGS2G5WLXKeZ-TdfVzWfYOpUhfS7MDvdtkNDew6_w1tTY14vuy7BomQ24u3aLHtwiqVDesueKQW--Sli8m9nZ9a0yqaXQADZ_9Arq7Nd1ckqfvw9WT-5d1CjNUaRCVV3ovIUB-ijgR4kosR6WksKmNNHrSpCqOsdpXNZNQ6THMnfSRy57yNkaNpYyYfwH7d1OER4KGz1EUpb4tKueiZs3hz5CyxK8JPO4FXW52V7ZCUo9ylX05SL0nqZZJ6aSbwYqvWkuYOS8bWodmsy0zTcjPj7dQJPBz0vRtPKibTUk_g9Va75Ti913952eN_6_4Ebig2kOQd-BT2-24TnsHe2m-ej1bN19Ozs9lPEXD1nQ |
| link.rule.ids | 315,782,786,27933,27934,41073,42142,48344,48347,49649,49652,52153 |
| linkProvider | Springer Nature |
| linkToHtml | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1NT9wwEB3BIrW9tNCWdkspU4lba4lNHBJzQ13QogIXQIKTZcc2rLRKomy2Ej-n_7QeJ1lUUVWCa_yRaMbOPHvezADsGqGdtWLEzJ7VjOvcMh27jGmhueFmFJng0Z1cpOfX2fiI0uTwPhYmsN17l2T4Uy-D3Wi9UTQxMXWyfc7EKqxRtvNoAGuH1zc34wdqB2-T7XrTyzhP0y5Y5t-z_G2QHqHMRx7SYHiO3zzvk9fhdQc08bBdGRuwYou38OKsc6W_g9_jrjRK0yoHc282c4_JsXRoQpAkVotZhZTpGel-H6dFU2Ko1YV-f-CdmtZtA5Hnb1EVvhF_TZu6RA_J1f0BKqxqOwuFw-p7mqnCJvB0MRDcadTcn6OJBGgp_wdSfW16OvOieg9Xx0eXPyasq9fA8pinDXNk7K1LnDd5MZUjSkYuy4USqU1EngmuEp2rKHZJYkepjo3z8E4b5RzF07oo3oRBURb2I-CeVr4L50ZlOdfOEGoxYl8rj6-8BVVD-NYrTVZtWg65TMAcpC691GWQuhRD-NrrVfrdQ5JRhS0Xcxkl_sAZ0YXqED60Cl_OF3OC03EyhO-9dmW3wef_edmnp3XfgZeTy7NTeXpy_nMLXkUUXRGIQZ9h0NQLuw2rc7P40i3xP2XM-B8 |
| linkToPdf | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1Rb9MwED6xTqr2AoNtUGBwSHtj1tbEWWLepnVlFVBN2pB4s-zYHpWqJEpTpP0c_ik-Jy2aipAQr7HjRHdn3WfffXcAR0ZoZ60YMnNqNeM6t0zHLmNaaG64GUYmRHSvbtLpt2x0SWVy1iz-kO2-Ckm2nAaq0lQ0J5VxJ2viG9keMYspayc740xswTZdi_EebJ9Pbj-Of6d58LbwrnfDjPM07Ygzf17loXPaQJwb0dLghMZP_v_3d-FxB0DxvLWYp_DIFs-g_6ULse_Bz1HXMqVplYa5d6e5x-pYOjSBPInVcl4hVYBGuvfHWdGUGHp4od83-F3N6naAkurvUBV-EH_MmrpED9XV_QdUWNV2HhqK1fe0UoVNyN_FkPhOby38-ZqSAy3VBUHqu01P515s-_B1fHl7ccW6Pg4sj3naMEcgwLrEeVcYU5uiZOiyXCiR2kTkmeAq0bmKYpckdpjq2DgP-7RRzhHP1kXxAfSKsrAvAE-18lM4NyrLuXaG0IwRZ1p53OU9qxrA-5UCZdWW65DrwsxB6tJLXQapSzGAdysdS7-rSDKqsOVyIaPEH0QjsqgBPG-Vv14v5gSz42QAxytNy27jL_7ysZf_Nv0t9K9HY_l5Mv30CnYiIl2EfKHX0GvqpT2ErYVZvums_ReF9wDF |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Differentiation+capacity+of+dental+pulp+stem+cell+into+inner+ear+hair+cell+using+an+in+vitro+assay%3A+a+preliminary+step+toward+treating+sensorineural+hearing+loss&rft.jtitle=European+archives+of+oto-rhino-laryngology&rft.au=Adriztina%2C+Indri&rft.au=Munir%2C+Delfitri&rft.au=Sandra%2C+Ferry&rft.au=Ichwan%2C+Muhamad&rft.date=2022-04-01&rft.pub=Springer+Berlin+Heidelberg&rft.issn=0937-4477&rft.eissn=1434-4726&rft.volume=279&rft.issue=4&rft.spage=1805&rft.epage=1812&rft_id=info:doi/10.1007%2Fs00405-021-06864-9&rft.externalDocID=10_1007_s00405_021_06864_9 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0937-4477&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0937-4477&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0937-4477&client=summon |