Isolation and characterization of the anthocyanidin genes PAL, F3H and DFR of Scutellaria viscidula (Lamiaceae)

• Published in: Genetics and molecular research Vol. 10; no. 4; pp. 3385 - 3402
• Main Authors: Lei, W, Yao, R X, Kang, X H, Tang, S H, Qiao, A M, Sun, M
• Format: Journal Article
• Language: English
• Published: Brazil 22-11-2011
• Subjects: Alcohol Oxidoreductases - chemistry; Alcohol Oxidoreductases - genetics; Alcohol Oxidoreductases - isolation & purification; Amino Acid Sequence; Anthocyanins - biosynthesis; Anthocyanins - genetics; Base Sequence; Biosynthetic Pathways - genetics; Cloning, Molecular; Conserved Sequence - genetics; DNA, Complementary - genetics; Evolution, Molecular; Genes, Plant - genetics; Mixed Function Oxygenases - chemistry; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - isolation & purification; Models, Molecular; Molecular Sequence Data; Phenylalanine Ammonia-Lyase - chemistry; Phenylalanine Ammonia-Lyase - genetics; Phenylalanine Ammonia-Lyase - isolation & purification; Phylogeny; Plant Proteins - chemistry; Plant Proteins - genetics; Protein Structure, Tertiary; Scutellaria - enzymology; Scutellaria - genetics; Sequence Alignment
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2011.November.22.7

Anthocyanidin is a group of flavonoid compounds used as a vegetable pigment and plays an important role in flower coloration and environmental adaptations of the Chinese ornamental plant Scutellaria viscidula. We determined the cDNA sequences of phenylalanine ammonia-lyase (SvPAL), flavanone 3-hydroxylase (SvF3H) and dihydroflavonol 4-reductase (SvDFR) genes in S. viscidula. Comparative analysis showed that the protein products of these three genes did not have a transit peptide at their N-terminal portion, which indicated that these enzymes were directly involved in the substrate conversion in the cytoplasmic matrix. Bioinformatic analysis further revealed that Svpal, Svf3h and Svdfr were the members of flavonoid biosynthetic genes with highly conserved motifs. Based on phylogenetic tree analysis, it appears that PAL, F3H or DFR from different plants might have originated from the same ancestor. This study can help to map and regulate the important stages involved in anthocyanidin biosynthesis by genetic engineering to diversify flower color and improve the ornamental value of S. viscidula.