The lack of attachment of transformed embryonic lung epithelial cells to collagen I is corrected by fibronectin and FXIII

• Published in: Journal of cell science Vol. 86; no. 1; pp. 95 - 107
• Main Authors: PAYE, M, LAPIERE, C. M
• Format: Journal Article
• Language: English
• Published: Cambridge Company of Biologists 01-12-1986
• Subjects: Animals; Biological and medical sciences; Cattle; Cell Adhesion; Cell Line - physiology; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; Cell Transformation, Neoplastic; Collagen - metabolism; Epithelium - embryology; Factor XIII - metabolism; Fibronectins - metabolism; Fundamental and applied biological sciences. Psychology; Lung - embryology; Molecular and cellular biology; Rats; Cell culture; Vertebrata; Regulation(control); Mammalia; Rat; Transformed cell; Collagen; Factor XIII; Rodentia; Adhesion; Coagulation factor; Fibronectin
• ISSN: 0021-9533; 1477-9137
• DOI: 10.1242/jcs.86.1.95

PER cells, a transformed pulmonary epithelial cell line that adhered to a large extent to a fibronectin substratum, were found to be attachment-deficient to collagen I. Although fibronectin can bind to collagen I monomers and polymers, the addition of exogenous fibronectin in the attachment medium induced the adhesion of these cells to collagen I polymers but not to monomers. By adding the transglutaminase of blood coagulation, FXIII, in the presence of fibronectin, the attachment of PER cells to collagen I monomers could be recovered while the minimal concentration of fibronectin needed to promote their adhesion to polymers was lowered. These studies indicate that FXIII enhances the fibronectin-mediated attachment of PER cells to collagen I.