Use of immunodiagnostic tests on an outbreak of scrapie in Latxa sheep: Pathogenetic and epidemiologic implications
A scrapie outbreak was detected in the year 2003 in a flock of 222 black-faced Latxa sheep when two suspect animals were submitted for laboratory diagnosis. After the two index cases were confirmed as scrapie positive by rapid tests and immunohistochemistry (IHC), two new clinical cases were recogni...
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| Published in: | Small ruminant research Vol. 72; no. 2; pp. 141 - 148 |
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| Abstract | A scrapie outbreak was detected in the year 2003 in a flock of 222 black-faced Latxa sheep when two suspect animals were submitted for laboratory diagnosis. After the two index cases were confirmed as scrapie positive by rapid tests and immunohistochemistry (IHC), two new clinical cases were recognized in the ante-mortem inspection during the flock destruction. Of the remaining 218 animals, 16 sheep were also positive to at least one of the rapid tests and IHC, and 6 more were negative by all rapid tests but positive by IHC in lymphoid and/or peripheral nervous tissues but not in central nervous system (CNS) tissues. Ten different genotypes were found in the flock, and, as expected, most of the positive sheep were of the ARQ/ARQ genotype (21/26, 80.7%), which was the most prevalent genotype in this flock and also in Latxa breed. In total, 26 positive ewes were identified in a flock with only 4 clinical cases (clinical/subclinical ratio of 1:5.5) indicating that the prevalence of infection in a positive flock might be considerably higher than clinical data indicates. The implications of the different patterns of PrP
Sc deposition in the pathogenesis and dissemination of scrapie within the host are discussed. |
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| AbstractList | A scrapie outbreak was detected in the year 2003 in a flock of 222 black-faced Latxa sheep when two suspect animals were submitted for laboratory diagnosis. After the two index cases were confirmed as scrapie positive by rapid tests and immunohistochemistry (IHC), two new clinical cases were recognized in the ante-mortem inspection during the flock destruction. Of the remaining 218 animals, 16 sheep were also positive to at least one of the rapid tests and IHC, and 6 more were negative by all rapid tests but positive by IHC in lymphoid and/or peripheral nervous tissues but not in central nervous system (CNS) tissues. Ten different genotypes were found in the flock, and, as expected, most of the positive sheep were of the ARQ/ARQ genotype (21/26, 80.7%), which was the most prevalent genotype in this flock and also in Latxa breed. In total, 26 positive ewes were identified in a flock with only 4 clinical cases (clinical/subclinical ratio of 1:5.5) indicating that the prevalence of infection in a positive flock might be considerably higher than clinical data indicates. The implications of the different patterns of PrP(Sc) deposition in the pathogenesis and dissemination of scrapie within the host are discussed. A scrapie outbreak was detected in the year 2003 in a flock of 222 black-faced Latxa sheep when two suspect animals were submitted for laboratory diagnosis. After the two index cases were confirmed as scrapie positive by rapid tests and immunohistochemistry (IHC), two new clinical cases were recognized in the ante-mortem inspection during the flock destruction. Of the remaining 218 animals, 16 sheep were also positive to at least one of the rapid tests and IHC, and 6 more were negative by all rapid tests but positive by IHC in lymphoid and/or peripheral nervous tissues but not in central nervous system (CNS) tissues. Ten different genotypes were found in the flock, and, as expected, most of the positive sheep were of the ARQ/ARQ genotype (21/26, 80.7%), which was the most prevalent genotype in this flock and also in Latxa breed. In total, 26 positive ewes were identified in a flock with only 4 clinical cases (clinical/subclinical ratio of 1:5.5) indicating that the prevalence of infection in a positive flock might be considerably higher than clinical data indicates. The implications of the different patterns of PrP Sc deposition in the pathogenesis and dissemination of scrapie within the host are discussed. |
| Author | Juste, R.A. Garrido, J.M. Garcia-Crespo, D. Gomez, N. Hurtado, A. Geijo, M.V. Benedicto, L. Korkostegi, J.L. |
| Author_xml | – sequence: 1 givenname: N. surname: Gomez fullname: Gomez, N. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 2 givenname: L. surname: Benedicto fullname: Benedicto, L. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 3 givenname: M.V. surname: Geijo fullname: Geijo, M.V. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 4 givenname: J.M. surname: Garrido fullname: Garrido, J.M. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 5 givenname: D. surname: Garcia-Crespo fullname: Garcia-Crespo, D. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 6 givenname: J.L. surname: Korkostegi fullname: Korkostegi, J.L. organization: Animal Husbandry Service, Diputacion Foral de Gipuzkoa, Plaza Gipuzkoa, s/n, 20004 Donostia-San Sebastián, Spain – sequence: 7 givenname: A. surname: Hurtado fullname: Hurtado, A. organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain – sequence: 8 givenname: R.A. surname: Juste fullname: Juste, R.A. email: rjuste@neiker.net organization: Department of Animal Health, NEIKER (Basque Institute for Agricultural Research and Development), Berreaga, 1, 48160 Derio, Bizkaia, Spain |
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| Cites_doi | 10.1136/vr.90.17.465 10.3168/jds.S0022-0302(93)77448-2 10.1099/vir.0.18825-0 10.1128/JCM.34.5.1228-1231.1996 10.1038/386564a0 10.1053/jcpa.1998.0300 10.1128/JCM.42.3.972-980.2004 10.1136/vr.154.23.717 10.1073/pnas.87.7.2476 10.1053/jcpa.2001.0476 10.1007/BF01718157 10.1128/JVI.78.7.3654-3662.2004 10.1099/0022-1317-80-11-3035 10.1099/0022-1317-80-7-1865 10.1034/j.1600-0463.2002.100104.x 10.1099/0022-1317-81-12-3115 10.1038/383685a0 10.1093/infdis/146.5.657 10.1354/vp.40-2-164 10.1073/pnas.95.23.13363 |
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| SubjectTerms | central nervous system disease detection disease outbreaks disease prevalence Encephalopaties ewes Genotype Immunohistochemistry Latxa (sheep breed) lymphatic system pathogenesis peripheral nervous system prions PrP PrPSc proteins rapid methods Rapid tests Scrapie sheep breeds tissue distribution TSE |
| Title | Use of immunodiagnostic tests on an outbreak of scrapie in Latxa sheep: Pathogenetic and epidemiologic implications |
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