Ergothioneine attenuates the DNA damage of post-thawed Merino ram sperm

• Published in: Small ruminant research Vol. 106; no. 2-3; pp. 165 - 167
• Main Authors: Çoyan, Kenan, Bucak, Mustafa Numan, Başpınar, Nuri, Taşpınar, Mehmet, Aydos, Sena
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-08-2012
• Subjects: antioxidant activity; DNA; DNA damage; DNA integrity; Ergothioneine; Freezing–thawing; Merino; Ram sperm; rams; semen; semen extenders; spermatozoa; Ergothioneine; DNA integrity; Ram sperm; Freezing–thawing
• ISSN: 0921-4488; 1879-0941
• DOI: 10.1016/j.smallrumres.2012.02.002

The objective of the current study was to evaluate the effects of antioxidant ergothioneine added to cryopreservation extender on the DNA integrity of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing ergothioneine at different concentrations and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed at 37°C for 20s in a water bath for evaluation of sperm DNA damage using the Comet test. The addition of ergothioneine at concentrations of 1, 2 and 4mM resulted in lower sperm with damaged DNA (5.4, 4.7 and 3.2%, respectively) than that of control (7.9%), thus reducing the DNA damage (P<0.01). Findings of this study showed that the increasing concentrations of ergothioneine in semen extenders, were of greater benefit to DNA integrity of frozen-thawed ram sperm.