Wheat- Thinopyrum Substitution Lines Imprint Compensation Both From Recipients and Donors

• Published in: Frontiers in plant science Vol. 13; p. 837410
• Main Authors: Lyu, Zhongfan, Hao, Yongchao, Chen, Liyang, Xu, Shoushen, Wang, Hongjin, Li, Mengyao, Ge, Wenyang, Hou, Bingqian, Cheng, Xinxin, Li, Xuefeng, Che, Naixiu, Zhen, Tianyue, Sun, Silong, Bao, Yinguang, Yang, Zujun, Jia, Jizeng, Kong, Lingrang, Wang, Hongwei
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 15-04-2022
• Subjects: compensation; distant hybridization; Plant Science; substitution line; Thinopyrum; wheat; distant hybridization; compensation; wheat; substitution line; Thinopyrum
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.837410

Even frequently used in wheat breeding, we still have an insufficient understanding of the biology of the products
distant hybridization. In this study, a transcriptomic analysis was performed for six
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substitution lines in comparison with the host plants. All the six disomic substitution lines showed much stronger "transcriptomic-shock" occurred on alien genomes with 57.43-69.22% genes changed expression level but less on the recipient genome (2.19-8.97%). Genome-wide suppression of alien genes along chromosomes was observed with a high proportion of downregulated genes (39.69-48.21%). Oppositely, the wheat recipient showed genome-wide compensation with more upregulated genes, occurring on all chromosomes but not limited to the homeologous groups. Moreover, strong co-upregulation of the orthologs between wheat and
sub-genomes was enriched in photosynthesis with predicted chloroplastic localization, which indicates that the compensation happened not only on wheat host genomes but also on alien genomes.