Study toward antioxidant activity of Clematis flammula extracts: Purification and identification of two flavonoids-glucoside and trisaccharide

• Published in: South African journal of botany Vol. 123; pp. 208 - 213
• Main Authors: Saidi, R., Chawech, R., Baccouch, N., Mezghani Jarraya, R.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-07-2019
• Subjects: Antioxidant; Clematis flammula; Flavonoid-glycoside; Trisaccharide; Flavonoid-glycoside; Antioxidant; Trisaccharide; Clematis flammula
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2019.03.010

The objective of this study was to investigate the antioxidant activity of Clematis flammula extracts and its main isolated compounds. The antioxidant tests were evaluated proceeding with DPPH-scavenging, reducing power (FRAP) and TAC-scavenging activity. The best antioxidant powers were given by leaf methanolic and hydro-alcoholic extracts with IC50 of 120 and 90 μg/mL respectively. The flower extracts IC50 were 410 and 190 μg/mL for the EtOAc and MeOH respectively. Fractionation of actives extracts led to the isolation of compounds identified to flavonoids glucosides 1 and 2 from MeOH and EtOAc flower residues and trisaccharide 3 from MeOH leaf extract. The present compounds showed antioxidant activities with IC50 of 20, 80 μg/mL and 70 μg/mL respectively. Results proved that flavonoid 1 exhibited higher antioxidant effects than 2. The antioxidant effects of main isolated molecules could be responsible of C. flammula antioxidant activity.
•The Tunisian Clematis flammula aerial parts showed remarkable antioxidant activity.•p-Coumaroyl moiety enhance antioxidant potential.•The bioguided extraction of leaves and flowers led to the isolation of three compounds.•The compounds isolated from C. flammula were detected for the first time.