Development and characterization of new microsatellites for Eugenia dysenterica DC (Myrtaceae)

Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymor...

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Bibliographic Details
Published in:	Genetics and molecular research Vol. 12; no. 3; pp. 3124 - 3127
Main Authors:	Telles, M P C, Silva, J B, Resende, L V, Vianello, R P, Chaves, L J, Soares, T N, Collevatti, R G
Format:	Journal Article
Language:	English
Published:	Brazil 06-02-2013
Subjects:	Alleles DNA, Plant - genetics Genetics, Population Genomic Library Microsatellite Repeats - genetics Polymorphism, Genetic Syzygium - genetics
Online Access:	Get full text
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Summary:	Microsatellite markers were developed for population genetic analyses of the Neotropical tree Eugenia dysenterica DC (Myrtaceae), after construction of a shotgun genomic library for microsatellite discovery. Nine primers were designed, of which 5 yielded amplified product. These primers were polymorphic for 97 individuals collected in 3 distinct localities. The number of alleles per locus (primer) ranged from 3 to 11 and expected heterozygosities varied from 0.309 to 0.884. The probability of locus identity was ~1.88 x 10(-4) and the probability of paternity exclusion was ~0.9367. The 5 microsatellite primer pairs may be suitable for population genetic studies such as parentage and fine-scale genetic analyses of this species.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1676-5680 1676-5680
DOI:	10.4238/2013.February.6.3