The effect of hypergravity on the lens, cornea and tail regeneration in Urodela

• Published in: Acta astronautica Vol. 138; pp. 423 - 433
• Main Authors: Grigoryan, E.N., Dvorochkin, N., Poplinskaya, V.A., Yousuf, R., Radugina, E.A., Almeida, E.A.
• Format: Journal Article
• Language: English
• Published: Elmsford Elsevier Ltd 01-09-2017; Elsevier BV
• Subjects: Animals; Centrifugation; Cornea; Detaching; Epithelium; Exposure; Eye; Eye (anatomy); Gravitational effects; Gravity; Hypergravity; Impact analysis; Intraocular pressure; Lens; Lenses; Microgravity; Newt; Regeneration; Restoration; Retina; Satellites; Space missions; Studies; Synchronism; Tail; Tissue engineering; Cornea; μCT; HSP; NR; RPE; PFA; IOP; Hypergravity; rpm; Newt; Regeneration; p/op; Tail; Lens; BrdU; PCR; ON
• ISSN: 0094-5765; 1879-2030
• DOI: 10.1016/j.actaastro.2017.06.013

In previous experiments onboard Russian Bion/Foton satellites it was found that exposure to microgravity causes changes in eye lens regeneration of Urodela. The changes included higher rate of regeneration, increased cell proliferation in lens anlage, and synchronization of lens restoration. Similar changes were observed regarding tail regeneration. Recently, investigations were performed to find out whether exposure to hypergravity could also alter lens, cornea and tail regeneration in the newt P. waltl. Nine days prior to exposure the left lens was surgically removed through corneal incision and distal 1/3 of the tail was amputated, thus initiating regeneration. The experimental animals were allowed to recover for 9 days at 1 g and then exposed to 2 g for 12 days in an 8 ft diameter centrifuge at NASA Ames Research Center. The experimental animals were divided into 1 g controls, 2 g centrifugation animals, basal controls, and aquarium controls. Lens and corneal regeneration appeared to be inhibited in 2 g group compared to 1 g animals. In all 1 g controls, lens regeneration reached stages VII-IX in a synchronous fashion and corneal regeneration was nearly complete. In the 2 g newts, neural retinal detachment from the pigmented epithelium was seen in most operated eyes. It was also observed in the non-operated (right) eyes of the animals exposed to 2 g. The level of retinal detachment varied and could have been caused by hypergravity-induced high intraocular pressure. Regeneration (when it could be assessed) proceeded asynchronously, reaching stages from II to IX. Corneal restoration was also noticeably delayed and corneal morphology changed. Cell proliferation was measured using BrdU; the results were not comparable to the 1 g data because of retinal detachment. Previous investigations demonstrated that lens regeneration was controlled by the neural retina; therefore, lower regeneration rate at 2 g was, at least in part, associated with retinal detachment. FGF2 release by the neural retina and FGFR2 expression in the iris and other tissues could accelerate lens proliferation whereas its delay could be caused by retinal detachment, which may explain compromised regeneration at 2 g. Hypergravity (both 1 g and 2 g) increased tissue growth compared to aquarium control (as measured by regenerate volume) and altered the shape of tail regenerates – they became curved downwards. The experimental results emphasize the important and versatile role gravity plays in tissue regeneration. They also suggest that, when considering hypergravity as a countermeasure that can be used in future space missions, its potential impact on the eye should not be ignored.
•Lens and corneal regeneration at 2 g was slowed down and abnormal.•Exposure to 2 g led to retinal detachment in both operated and control eyes.•FGF2-FGFR2 presence in eye tissues was correlated with the progress of regeneration.•Increased size and altered shape of tail regenerates were observed at both 1 g and 2 g.