Changes in Na+, K+-ATPase Activity and Alpha 3 Subunit Expression in CNS After Administration of Na+, K+-ATPase Inhibitors

• Published in: Neurochemical research Vol. 36; no. 2; pp. 297 - 303
• Main Authors: Bersier, María Geraldina, Peña, Clara, Rodríguez de Lores Arnaiz, Georgina
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-02-2011
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Central Nervous System - drug effects; Central Nervous System - metabolism; Enzyme Inhibitors - pharmacology; Male; Neurochemistry; Neurology; Neurosciences; Original Paper; Ouabain - analogs & derivatives; Ouabain - pharmacology; Rats; Rats, Wistar; Sodium-Potassium-Exchanging ATPase - antagonists & inhibitors; Sodium-Potassium-Exchanging ATPase - metabolism; Synaptosomes - drug effects; Synaptosomes - enzymology; Endobain E; Na; ATPase activity; Expression; ATPase; Ouabain; ATPase inhibitors; K; α3 Subunit
• DOI: 10.1007/s11064-010-0317-x
• EISSN: 1573-6903
• ISSN: 0364-3190

The expression of Na
+
, K
+
-ATPase α3 subunit and synaptosomal membrane Na
+
, K
+
-ATPase activity were analyzed after administration of ouabain and endobain E, respectively commercial and endogenous Na
+
, K
+
-ATPase inhibitors. Wistar rats received intracerebroventricularly ouabain or endobain E dissolved in saline solution or Tris–HCl, respectively or the vehicles (controls). Two days later, animals were decapitated, cerebral cortex and hippocampus removed and crude and synaptosomal membrane fractions were isolated. Western blot analysis showed that Na
+
, K
+
-ATPase α3 subunit expression increased roughly 40% after administration of 10 or 100 nmoles ouabain in cerebral cortex but remained unaltered in hippocampus. After administration of 10 μl endobain E (1 μl = 28 mg tissue) Na
+
, K
+
-ATPase α3 subunit enhanced 130% in cerebral cortex and 103% in hippocampus. The activity of Na
+
, K
+
-ATPase in cortical synaptosomal membranes diminished or increased after administration of ouabain or endobain E, respectively. It is concluded that Na
+
, K
+
-ATPase inhibitors modify differentially the expression of Na
+
, K
+
-ATPase α3 subunit and enzyme activity, most likely involving compensatory mechanisms.