Detecting platform for phenolic compounds–characteristic of enzymatic electrode

• Published in: Optical materials Vol. 34; no. 10; pp. 1677 - 1681
• Main Authors: Cabaj, Joanna, Chyla, Antoni, Jędrychowska, Agnieszka, Olech, Kamila, Sołoducho, Jadwiga
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-08-2012
• Subjects: AFM imaging; Biosensing effect; Conducting copolymer; Enzymatic electrode; Tyrosinase; Enzymatic electrode; Biosensing effect; Conducting copolymer; Tyrosinase; AFM imaging
• ISSN: 0925-3467; 1873-1252
• DOI: 10.1016/j.optmat.2012.02.042

► We model an enzymatic electrode built as hybrid protein-copolymer on Pt electrode. ► We modify the Pt electrodes with conducting copolymer before enzyme deposition. ► We optimize the copolymer synthesis. ► We perform the immobilization of tyrosinase in one step process. ► We built stable and active sensoric device.
We report here on simple and universal method for the highly efficient, electrolytic immobilization of tyrosinase (from Agaricus bisporus), for amperometric biosensing purposes. Tyrosinase has been successfully deposited on the surface of thin, ordered films of copolymerized derivative of thiophene (3-methylthiophene/3-thiopheneacetic acid/bis(ethylenedioxythiophene)diphenylamine). The tyrosinase retains well its activity well within the fabricated copolymer matrix. Reduction peaks, observed in cyclic voltammetry at 0.125 to +0.07V, were attributed to the reduction of enzymatically liberated quinone species. Considering the fact, that immobilization strategy showed high efficiency, obtained results suggest that the method for phenoloxidase immobilization has a great potential for fabrication of bioelectronics’ devices.