Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1397; p. 155
• Main Authors: Small, David M, Sanchez, Washington Y, Gobe, Glenda C
• Format: Journal Article
• Language: English
• Published: United States 2016
• Subjects: Acute Kidney Injury - etiology; Acute Kidney Injury - metabolism; Acute Kidney Injury - pathology; Animals; Disease Models, Animal; Kidney - cytology; Kidney - metabolism; Kidney Function Tests; Kidney Tubules - cytology; Kidney Tubules - metabolism; Male; Mice; Microscopy, Fluorescence, Multiphoton - methods; Mitochondria - metabolism; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Fluorescence; Intravital imaging; Multiphoton; Metabolism; Two-photon excitation
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-3353-2_12

Multiphoton microscopy (MPM) allows the visualization of dynamic pathophysiological events in real time in live animals. Intravital imaging can be applied to investigate novel mechanisms and treatments of different forms of kidney disease as well as improve our understanding of normal kidney physiology. Using rodent models, in conjunction with endogenous fluorescence and infused exogenous fluorescent dyes, measurement can be made of renal processes such as glomerular permeability, juxtaglomerular apparatus function, interactions of the tubulointerstitium, tubulovascular interactions, vascular flow rate, and the renin-angiotensin-aldosterone system. Subcellular processes including mitochondrial dynamics, reactive oxygen species production, cytosolic ion concentrations, and death processes of apoptosis and necrosis can also be seen and measured by MPM. The current methods chapter presents an overview of MPM with a focus on techniques for intravital kidney imaging and gives examples of instances where intravital MPM has been utilized to study renal pathophysiology. Suggestions are provided for MPM methods within the confines of intravital microscopy and selected kidney structure. MPM is undoubtedly a powerful new technique for application in experimental nephrology, and we believe it will continue to create new paradigms for understanding and treating kidney disease.