Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads

Alginate Encapsulation of Human Hepatocytes and Assessment of Microbeads

Alginate encapsulation of cells is an attractive technique in which alginate becomes polymerized entrapping the cells. The structure of formed microbeads/microcapsules is semipermeable as it allows oxygen and nutrients to go in, and waste products and other materials produced by the cells to go out....

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Bibliographic Details
Published in:Methods in molecular biology (Clifton, N.J.) Vol. 1506; p. 273
Main Authors: Mitry, Ragai R, Jitraruch, Suttiruk, Iansante, Valeria, Dhawan, Anil
Format: Journal Article
Language:English
Published: United States 2017
Subjects:
Alginates - chemistry
Capsules - chemistry
Cell Culture Techniques
Cell Separation - methods
Cell Survival
Cell Transplantation - instrumentation
Cell Transplantation - methods
Colorimetry - methods
Drug Compounding - instrumentation
Drug Compounding - methods
Enzyme-Linked Immunosorbent Assay - methods
Glucuronic Acid - chemistry
Hepatocytes - physiology
Hepatocytes - transplantation
Hexuronic Acids - chemistry
Humans
Liver - cytology
Liver - surgery
Microscopy
Microspheres
Encapsulation
Microbeads
Microcapsules
Alginate
Hepatocyte
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Summary:Alginate encapsulation of cells is an attractive technique in which alginate becomes polymerized entrapping the cells. The structure of formed microbeads/microcapsules is semipermeable as it allows oxygen and nutrients to go in, and waste products and other materials produced by the cells to go out. Here, we describe basic protocols for alginate encapsulation of human hepatocytes and methods for assessing the microbeads produced.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6506-9_19