Antimicrobial nanofibrous mats with controllable drug release produced from hydrophobized hyaluronan

• Published in: Carbohydrate polymers Vol. 267; p. 118225
• Main Authors: Bardoňová, Lenka, Kotzianová, Adéla, Skuhrovcová, Kristýna, Židek, Ondřej, Bártová, Tereza, Kulhánek, Jaromír, Hanová, Tereza, Mamulová Kutláková, Kateřina, Vágnerová, Hana, Krpatová, Věra, Knor, Michal, Starigazdová, Jana, Holomková, Pavlína, Buffa, Radovan, Velebný, Vladimír
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-09-2021
• Subjects: Electrospinning; Lauroyl hyaluronan; Nanofiber; Octenidine; Triclosan; PBS; OCT; MO; PLA; EDC; MIC; Electrospinning; Nanofiber; TRI; ES; HV; DW; PLGA; Octenidine; PCL; HA; L-HA; Lauroyl hyaluronan; API; PVA; Triclosan; IPA; PEO
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2021.118225

Due to their large active surface, high loading efficiency, and tunable dissolution profiles, nanofibrous mats are often cited as promising drug carriers or antimicrobial membranes. Hyaluronic acid has outstanding biocompatibility, but it is hydrophilic. Nanofibrous structures made from hyaluronan dissolve immediately, making them unsuitable for controlled drug release and longer applications. We aimed to prepare a hyaluronan-based antimicrobial nanofibrous material, which would retain its integrity in aqueous environments. Self-supporting nanofibrous mats containing octenidine dihydrochloride or triclosan were produced by electrospinning from hydrophobized hyaluronan modified with a symmetric lauric acid anhydride. The nanofibrous mats required no cross-linking to be stable in PBS for 7 days. The encapsulation efficiency of antiseptics was nearly 100%. Minimal release of octenidine was observed, while up to 30% of triclosan was gradually released in 72 h. The nanofibrous materials exhibited antimicrobial activity, the fibroblast viability was directly dependent on the antiseptic content and its release.
•Self-supporting nanofibrous materials from hydrophobized hyaluronan derivative were prepared by electrospinning.•No degradation of API was observed due to high voltage, materials exhibit antibacterial effects against different bacteria.•High encapsulation efficiency of both API was achieved as the process parameters were optimized.•Hydrophobized form of hyaluronan ensured mechanical stability after submerged in water-based solutions.•Using a needleless multi-jet spinning nozzle, the production process could be up-scaled from laboratory to production line.