HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments
This study analyzed field and acclimatized (7 days) mangrove Ucides cordatus crabs from polluted and unpolluted environments to compare their HSP70 levels. The animals were cryo-anesthetized and dissected. Gills (anterior and posterior) and hepatopancreas were collected to evaluate total proteins an...
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| Published in: | Ecologies (Basel) Vol. 3; no. 3; pp. 249 - 256 |
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| Abstract | This study analyzed field and acclimatized (7 days) mangrove Ucides cordatus crabs from polluted and unpolluted environments to compare their HSP70 levels. The animals were cryo-anesthetized and dissected. Gills (anterior and posterior) and hepatopancreas were collected to evaluate total proteins and HSP70 levels using ELISA (Enzyme-Linked Immunosorbent Assay) method. The acclimatized animals from polluted environments showed higher HSP70 levels in the hepatopancreas than field animals. Results showed higher HSP70 levels in laboratory animals from the polluted environment than in field animals in the posterior gills. The regulation to decrease the damage caused by the environment and the acclimatization process may not be sufficient to stabilize physiological responses, especially in animals from polluted environments. |
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| AbstractList | This study analyzed field and acclimatized (7 days) mangrove Ucides cordatus crabs from polluted and unpolluted environments to compare their HSP70 levels. The animals were cryo-anesthetized and dissected. Gills (anterior and posterior) and hepatopancreas were collected to evaluate total proteins and HSP70 levels using ELISA (Enzyme-Linked Immunosorbent Assay) method. The acclimatized animals from polluted environments showed higher HSP70 levels in the hepatopancreas than field animals. Results showed higher HSP70 levels in laboratory animals from the polluted environment than in field animals in the posterior gills. The regulation to decrease the damage caused by the environment and the acclimatization process may not be sufficient to stabilize physiological responses, especially in animals from polluted environments. |
| Author | Ortega, Priscila Vitorino, Hector Aguilar |
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| Cites_doi | 10.1016/j.neuro.2019.10.008 10.1002/(SICI)1097-010X(19971001)279:2<118::AID-JEZ2>3.0.CO;2-R 10.1016/j.chemosphere.2006.02.042 10.1016/j.aquatox.2017.11.020 10.1016/0003-2697(76)90527-3 10.1007/s10661-013-3172-9 10.1016/j.jembe.2008.02.006 10.1007/978-3-319-73377-7_10 10.1186/s13002-017-0174-7 10.1007/s12192-013-0402-6 10.1007/978-94-011-2304-4_12 10.1016/j.aquatox.2010.10.001 10.1016/j.ecoenv.2016.07.018 10.1016/j.heliyon.2020.e04691 10.1002/etc.3537 10.1371/journal.pone.0174762 10.1007/s11356-015-4398-6 10.1590/S1679-87592010000200006 10.1016/j.cbpc.2009.07.002 10.1016/j.aquatox.2011.03.001 10.1007/s12601-013-0017-y 10.1007/s002510000233 10.1016/j.cbpc.2021.109210 10.1016/j.scitotenv.2019.06.039 10.1016/S1050-4648(03)00030-5 10.1016/j.envint.2014.05.018 10.1098/rsos.171802 10.1111/j.1365-294X.2007.03522.x 10.1007/978-3-030-35691-0_1 10.1006/eesa.1997.1595 10.1007/s11270-010-0589-4 |
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| SubjectTerms | acclimatization Animals crabs Crustaceans Heat shock proteins HSP70 Laboratories Metals Physiology Pollution Sediments Ucides cordatus |
| Title | HSP70 in Gills and Hepatopancreas of Mangrove Crabs Ucides cordatus: Comparison between Contaminated and Pristine Environments |
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