Loss of antibody binding to prefixed cells: fixation parameters for immunocytochemistry

The denaturing effects of various types of fixative solutions on 5 cell surface antigens on mouse T-lymphocytes (Thy-1, T-200, Lyt-1, Lyt-2 and Th-B) were studied. For this purpose, cells were fixed with paraformaldehyde, glutaraldehyde, acrolein and osmium tetroxide at various concentrations. Fixed...

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Bibliographic Details
Published in:	The Histochemical journal Vol. 16; no. 2; p. 179
Main Authors:	Van Ewijk, W, Van Soest, P L, Verkerk, A, Jongkind, J F
Format:	Journal Article
Language:	English
Published:	Netherlands 01-02-1984
Subjects:	Acrolein - pharmacology Animals Antibodies, Monoclonal - immunology Binding Sites, Antibody - drug effects Enzyme-Linked Immunosorbent Assay Fixatives - pharmacology Flow Cytometry Formaldehyde - pharmacology Glutaral - pharmacology Male Mice Mice, Inbred BALB C Osmium Tetroxide - pharmacology Polymers - pharmacology T-Lymphocytes - drug effects T-Lymphocytes - immunology Thymus Gland - cytology
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