Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid

The production and characterization of high-affinity monoclonal antibodies suitable for the radio- and enzymeimmunoassay of the endogenous plant growth regulator, indole-3-acetic acid (IAA), is reported. Hybridomas were produced by fusion of NS 1 myeloma cells with spleen cells from Balb/c mice immu...

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Bibliographic Details
Published in:Planta Vol. 166; no. 3; pp. 389 - 393
Main Authors: Mertens, R, Eberle, J, Arnscheidt, A, Ledebur, A, Weiler, E.W
Format: Journal Article
Language:English
Published: Berlin Springer-Verlag 01-11-1985
Springer
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Summary:The production and characterization of high-affinity monoclonal antibodies suitable for the radio- and enzymeimmunoassay of the endogenous plant growth regulator, indole-3-acetic acid (IAA), is reported. Hybridomas were produced by fusion of NS 1 myeloma cells with spleen cells from Balb/c mice immunized with IAA-bovine serum albumin conjugates. From an initial collection of 158 wells containing cells secreting monoclonal antibodies against IAA, seven were used to derive cell clones. Three of these are described here. They secrete immunoglobulin (IgG2a or IgG2b) of high affinity and specificity for IAA methyl ester and can be used to quantitate picogram amounts of this compound in plant extracts by radio- and enzymeimmunoassay.
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ISSN:0032-0935
1432-2048
DOI:10.1007/bf00401177