Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum
OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase an...
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| Published in: | The Brazilian journal of infectious diseases Vol. 15; no. 5; pp. 442 - 448 |
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| Language: | English |
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Brazilian Society of Infectious Diseases
01-10-2011
Elsevier |
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| Abstract | OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism. |
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| AbstractList | OBJECTIVES: Apoptosis is the process of programmed cell death (PCD) that occurs in both animal and plant cells. Protozoan parasites possess metacaspase and these caspase-related proteases could be involved in the PCD pathways in these organisms. Therefore we analyzed the activities of metacaspase and PARP genes in Leishmania infantum (MCAN/IR/96/LON49) treated with miltefosine. MATERIALS AND METHODS: Anti-leishmania activity of miltefosine was studied by treatment of cultured promastigotes with various concentration of miltefosine. MTT assay and Annexin-V FLUOS staining by using FACS flow cytometry methods were used. Cytotoxic potential of HePC on the amastigots of L.infantum was evaluated in J774 cell line. In addition, metacaspase and PARP genes expression of treated L. infantum were studied. RESULTS: Miltefosine led to dose-dependent death of L. infantumwith features compatible with apoptosis. Over expression of metacaspase and PARP was seen 6 hr after treatment. CONCLUSIONS: Our study showed that miltefosine exerts cytotoxic effect on L. infantum via an apoptotic-related mechanism. |
| Author | Gharavi, Mohammad Javad Saki, Jasem Khademvatan, Shahram |
| AuthorAffiliation | Tehran University of Medical Sciences Ahvaz Jundishapur University of Medical Sciences |
| AuthorAffiliation_xml | – name: Ahvaz Jundishapur University of Medical Sciences – name: Tehran University of Medical Sciences |
| Author_xml | – sequence: 1 givenname: Shahram surname: Khademvatan fullname: Khademvatan, Shahram – sequence: 2 givenname: Mohammad Javad surname: Gharavi fullname: Gharavi, Mohammad Javad – sequence: 3 givenname: Jasem surname: Saki fullname: Saki, Jasem |
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| Keywords | apoptosis Leishmania infantum gene expression |
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| Title | Miltefosine induces metacaspase and PARP genes expression in Leishmania infantum |
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