Woronin bodies in Penicillium janczewskii Zaleski
Penicillium janczewskii Zaleski is an efficient microorganism for the production of extracellular inulinases and grows rapidly on medium containing sucrose or inulin as carbon source. Maintenance of this filamentous fungus on inulin medium induces secretion of large amounts of inulinases, but the re...
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Published in: | Brazilian journal of microbiology Vol. 33; no. 2; pp. 127 - 130 |
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Sociedade Brasileira de Microbiologia
01-06-2002
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Abstract | Penicillium janczewskii Zaleski is an efficient microorganism for the production of extracellular inulinases and grows rapidly on medium containing sucrose or inulin as carbon source. Maintenance of this filamentous fungus on inulin medium induces secretion of large amounts of inulinases, but the resulting mycelium has thinner cell walls that easily collapse and break. Woronin bodies in hyphae of P. janczewskii grown on sucrose and inulin substrates were observed. No significant differences in the number, location, size and shape of Woronin bodies and level of plugging were observed in cultures of the fungus grown on the two carbon sources. The data indicate that the presence of Woronin bodies in P. janczewskii could not be associated with more easily damaged hyphae, although the function of these organelles in pore plugging has been confirmed.
Penicillium janczewskii Zaleski é um microrganismo eficiente para a produção de inulisases extracelulares e cresce rapidamente em meio contendo sagarose ou inulina como fonte de carbono. A manutenção desse fungo filamentoso em meio com inulina induz a secreção de grandes quantidades de inulisanes, mas resulta na presença de um micélio com parede celulares mais finas, que facilmente colapsa e se danifica. A presença de corpúsculos de Woronin foi analisada nas hifas de P. janczewskii crescido em sacarose e inulina. Nenhuma diferença significativa foi observada no número, localização, tamanho e forma dos corpúsculos de Woronin e na obstrução dos poros dos septos nas culturas do fungo crescido nas duas diferentes fontes de carbono. Os resultados obtidos indicam que a presença dos corpúsculos de Woronin em P. janczewskii não pode ser associada com a presença de hifas mais facilmente danificadas, embora a função dessas organelas na obstrução dos poros dos septos tenha sido confirmada. |
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AbstractList | Penicillium janczewskii Zaleski is an efficient microorganism for the production of extracellular inulinases and grows rapidly on medium containing sucrose or inulin as carbon source. Maintenance of this filamentous fungus on inulin medium induces secretion of large amounts of inulinases, but the resulting mycelium has thinner cell walls that easily collapse and break. Woronin bodies in hyphae of P. janczewskii grown on sucrose and inulin substrates were observed. No significant differences in the number, location, size and shape of Woronin bodies and level of plugging were observed in cultures of the fungus grown on the two carbon sources. The data indicate that the presence of Woronin bodies in P. janczewskii could not be associated with more easily damaged hyphae, although the function of these organelles in pore plugging has been confirmed. Penicillium janczewskii Zaleski is an efficient microorganism for the production of extracellular inulinases and grows rapidly on medium containing sucrose or inulin as carbon source. Maintenance of this filamentous fungus on inulin medium induces secretion of large amounts of inulinases, but the resulting mycelium has thinner cell walls that easily collapse and break. Woronin bodies in hyphae of P. janczewskii grown on sucrose and inulin substrates were observed. No significant differences in the number, location, size and shape of Woronin bodies and level of plugging were observed in cultures of the fungus grown on the two carbon sources. The data indicate that the presence of Woronin bodies in P. janczewskii could not be associated with more easily damaged hyphae, although the function of these organelles in pore plugging has been confirmed. Penicillium janczewskii Zaleski é um microrganismo eficiente para a produção de inulisases extracelulares e cresce rapidamente em meio contendo sagarose ou inulina como fonte de carbono. A manutenção desse fungo filamentoso em meio com inulina induz a secreção de grandes quantidades de inulisanes, mas resulta na presença de um micélio com parede celulares mais finas, que facilmente colapsa e se danifica. A presença de corpúsculos de Woronin foi analisada nas hifas de P. janczewskii crescido em sacarose e inulina. Nenhuma diferença significativa foi observada no número, localização, tamanho e forma dos corpúsculos de Woronin e na obstrução dos poros dos septos nas culturas do fungo crescido nas duas diferentes fontes de carbono. Os resultados obtidos indicam que a presença dos corpúsculos de Woronin em P. janczewskii não pode ser associada com a presença de hifas mais facilmente danificadas, embora a função dessas organelas na obstrução dos poros dos septos tenha sido confirmada. |
Author | Freshour, Glenn(University of Georgia) Hahn, Michael G.(University of Georgia) Pessoni, Rosemeire A.B.(Universidade Metodista de São Paulo) Figueiredo-Ribeiro, Rita de Cássia L.(Instituto de Botânica Seção de Fisiologia e Bioquímica de Plantas) Braga, Márcia R.(Instituto de Botânica Seção de Fisiologia e Bioquímica de Plantas) |
AuthorAffiliation | Universidade Metodista de São Paulo University of Georgia Instituto de Botânica |
AuthorAffiliation_xml | – name: Instituto de Botânica – name: University of Georgia – name: Universidade Metodista de São Paulo |
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Keywords | inulin Woronin bodies Penicilliun sacarose Penicillium inulina sucrose corpúsculos de Woronin |
Language | English |
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References | Collinge, A.J.; Markham, P. 1992; 91 Cordeiro Neto, F.; Pessoni, R.A.B.; Figueiredo-Ribeiro, R.C.L. 1997; 21 Pandey, A.; Soccol, C.R.; Selvakumar, P.; Soccol, V.T.; Krieger, N.; Fontana, J.D. 1999; 81 Reynolds, E.S. 1963; 17 Turnau, K.; Kottke, I.; Oberwinkler, F. 1993; 97 Pessoni, R.A.B.; Figueiredo-Ribeiro, R.C.L.; Braga, M.R. 1999; 86 Markham, P. 1994; 98 Rowley, J.C. III; Moran, D.T. 1975; 1 Larrondo, J.V.; Calvo, M.A. 1992; 119 Jedd, G.; Chua, N. 2000; 2 |
References_xml | – volume: 17 start-page: 208 year: 1963 end-page: 212 article-title: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy publication-title: J. Cell. Biol. contributor: fullname: Reynolds, E.S. – volume: 2 start-page: 226 year: 2000 end-page: 231 article-title: A new self-assembled peroxisomal vesicle required for efficient resealing of the plasma membrane publication-title: Nat. Cell. Biol. contributor: fullname: Jedd, G.; Chua, N. – volume: 97 start-page: 1499 year: 1993 end-page: 1504 article-title: Comparative study of elongated and globose Woronin bodies using electron energy loss spectroscopy (EELS) and imaging (ESI) publication-title: Mycol. Res. contributor: fullname: Turnau, K.; Kottke, I.; Oberwinkler, F. – volume: 86 start-page: 141 year: 1999 end-page: 147 article-title: Extracellular inulinases from Penicillium janczewskii, a fungus isolated from the rhizosphere of Vernonia herbacea (Asteraceae) publication-title: J. App. Microbiol. contributor: fullname: Pessoni, R.A.B.; Figueiredo-Ribeiro, R.C.L.; Braga, M.R. – volume: 119 start-page: 157 year: 1992 end-page: 160 article-title: Woronin bodies in Arthrinium genus publication-title: Mycopathologia contributor: fullname: Larrondo, J.V.; Calvo, M.A. – volume: 91 start-page: 49 year: 1992 end-page: 54 article-title: Ultrastructure of hyphal tip bursting in Penicillium chrysogenum publication-title: FEMS Microbiol. Lett. contributor: fullname: Collinge, A.J.; Markham, P. – volume: 1 start-page: 151 year: 1975 end-page: 155 article-title: A simple procedure for mounting wrinkle-free sections on Formvar-coated slot grids publication-title: Ultramicroscopy. contributor: fullname: Rowley, J.C. III; Moran, D.T. – volume: 98 start-page: 1089 year: 1994 end-page: 1106 article-title: Occlusions of septal pores in filamentous fungi publication-title: Mycol. Res. contributor: fullname: Markham, P. – volume: 81 start-page: 35 year: 1999 end-page: 52 article-title: Recent developments in microbial inulinases publication-title: App. Biochem. Biotechnol. contributor: fullname: Pandey, A.; Soccol, C.R.; Selvakumar, P.; Soccol, V.T.; Krieger, N.; Fontana, J.D. – volume: 21 start-page: 149 year: 1997 end-page: 153 article-title: Fungos produtores de inulinases isolados da rizosfera de asteráceas herbáceas do cerrado publication-title: Rev. Bras. Ci. Solo contributor: fullname: Cordeiro Neto, F.; Pessoni, R.A.B.; Figueiredo-Ribeiro, R.C.L. |
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SubjectTerms | corpúsculos de Woronin inulin inulina MICROBIOLOGY Penicillium Penicilliun sacarose sucrose Woronin bodies |
Title | Woronin bodies in Penicillium janczewskii Zaleski |
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