Analyses of relative protein content and distribution of histones in euchromatic and heterochromatic fractions

Analyses of relative protein content and distribution of histones in euchromatic and heterochromatic fractions

Euchromatic and heterochromatic fractions obtained by autodigestion of mouse TLT (taper liver tumour) hepatoma chromatin (Paul, I. J & Duerksen, J. D. (1976) Arch. Biochem. Biophys. 174, 491-505) were analyzed for relative protein content and histone content. With one exception, all fractions ha...

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Bibliographic Details
Published in:Canadian journal of biochemistry Vol. 55; no. 11; p. 1140
Main Authors: Paul, I J, Duerksen, J D
Format: Journal Article
Language:English
Published: Canada 01-11-1977
Subjects:
Animals
Calcium
Carcinoma, Hepatocellular - analysis
Chromatin - analysis
DNA - analysis
Heterochromatin - analysis
Histones - analysis
Liver Neoplasms - analysis
Magnesium
Mice
Proteins - analysis
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Summary:Euchromatic and heterochromatic fractions obtained by autodigestion of mouse TLT (taper liver tumour) hepatoma chromatin (Paul, I. J & Duerksen, J. D. (1976) Arch. Biochem. Biophys. 174, 491-505) were analyzed for relative protein content and histone content. With one exception, all fractions had the same DNA to protein ratio. Similarly, the total histone to DNA ratio was also constant in all fractions. In addition, the relative contents of the major histones, H1, (H2A + H2B + H3), and H4, were also constant in all fractions.
ISSN:0008-4018
DOI:10.1139/o77-170