Resonance Raman Evidence of Immobilization of Laccase on Self-Assembled Monolayers of Thiols on Ag and Au Surfaces

• Published in: Applied spectroscopy Vol. 60; no. 7; pp. 752 - 757
• Main Authors: Michota-Kaminska, A., Wrzosek, B., Bukowska, J.
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01-07-2006
• Subjects: 3-Mercaptopropionic Acid; Biosensing Techniques; Enzymes, Immobilized - chemistry; Gold - chemistry; Laccase - chemistry; Oxygen - chemistry; Silver - chemistry; Spectrum Analysis, Raman; Sulfhydryl Compounds - chemistry; Resonance Raman; Surface-enhanced Raman scattering; SERS; Laccase immobilization
• ISSN: 0003-7028; 1943-3530
• DOI: 10.1366/000370206777887035

Resonance Raman (RR) and surface-enhanced Raman scattering (SERS) spectroscopy have been used to study immobilization of laccase on self-assembled monolayers (SAMs) of thiols containing carboxylic and amino groups, deposited on silver and gold electrodes. A new, indirect way of monitoring laccase bound to the thiol-coated Ag and Au surfaces is presented. It was demonstrated that by recording the resonance Raman spectra of the colored product of the oxidation of syringaldazine (4-hydroxy-3,5-dimethoxybenzaldehydeazine) by laccase in the presence of molecular oxygen, one may easily confirm binding as well as enzymatic activity of laccase immobilized on the SAMs modified silver and gold surfaces.