Construction of Multicopy Expression Vectors for Regulated Over-production of Proteins in Klebsiella pneumoniae and Other Enteric Bacteria

AFRC-IPSR Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, UK ABSTRACT Summary: A number of expression vectors have been constructed to allow over-production of selected gene products in Klebsiella pneumoniae and other enteric bacteria. The plasmids use the strong hybrid trp-lac...

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Bibliographic Details
Published in:	Journal of general microbiology Vol. 134; no. 7; pp. 1779 - 1784
Main Authors:	Kleiner, Diethelm, Paul, Wyatt, Merrick, Mike J
Format:	Journal Article
Language:	English
Published:	London Soc General Microbiol 01-07-1988 New York, NY Cambridge University Press
Subjects:	Bacterial Proteins - biosynthesis Bacteriology Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genes, Bacterial Genetic engineering Genetic technics Genetics Klebsiella pneumoniae - genetics Klebsiella pneumoniae - metabolism Methods. Procedures. Technologies Microbiology Plasmids Promoter Regions, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons Genetic mapping Enzymatic synthesis Gene dosage Transcription promoter Activation Gene expression Genetic transfer Plasmid Proteins Regulation(control) Production Bacteria Molecular cloning Vector Klebsielleae Hybrid gene Klebsiella pneumoniae Enterobacteriaceae
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