Sensitivity of multiplex real-time PCR reactions, using the LightCycler and the ABI PRISM 7700 Sequence Detection System, is dependent on the concentration of the DNA polymerase

The introduction of multiplex PCR techniques to clinical laboratories has provided a means to streamline assays and to produce multiple results with minimal effort. While this methodology is very beneficial, care must be taken to ensure that reactions are properly optimized to allow for maximum sens...

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Bibliographic Details

Published in:	Molecular and cellular probes Vol. 16; no. 5; pp. 351 - 357
Main Authors:	Exner, M.M., Lewinski, M.A.
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-10-2002
Subjects:	Cytomegalovirus - genetics DNA - analysis DNA-Directed DNA Polymerase HLA-DQ Antigens - genetics Humans multiplex PCR, real-time PCR, DNA polymerase, LightCycler, ABI PRISM 7700 Polymerase Chain Reaction - instrumentation Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards Reagent Kits, Diagnostic Sensitivity and Specificity Sequence Analysis, DNA - methods Sequence Analysis, DNA - standards multiplex PCR, real-time PCR, DNA polymerase, LightCycler, ABI PRISM 7700
Online Access:	Get full text
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