Assessment of Cell Disruption and Carotenoids Extraction from Sporidiobolus salmonicolor (CBS 2636)

• Published in: Food and bioprocess technology Vol. 2; no. 2; pp. 234 - 238
• Main Authors: Valduga, Eunice, Valério, Alexsandra, Tatsch, Pihetra Oliveira, Treichel, Helen, Furigo, Agenor Jr, Di Luccio, Marco
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01-06-2009; Springer Nature B.V
• Subjects: Acetic acid; Acetone; Agriculture; biobased products; Biotechnology; Carotenoids; Cell disruption; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Chloroform; Communication; Cultivation; Diatomaceous earth; Dimethyl sulfoxide; Ethyl acetate; extraction; extracts; Food Science; food technology; Intracellular; Petroleum ether; Pigments; Solvents; Sporidiobolus; Sporidiobolus salmonicolor; Sulfoxides; Synergistic effect; yeast extract; Recovery; Carotenoids; Disruption
• ISSN: 1935-5149; 1935-5130; 1935-5149
• DOI: 10.1007/s11947-008-0133-3

The increasing demand for carotenoids by industries has drawn attention to their bio-production. Since pigments are intracellular, extraction steps are then needed after cell cultivation. In this work, different strategies for extraction of carotenoid pigments from Sporidiobolus salmonicolor (CBS 2636) were studied. Different solvents (dimethyl sulfoxide, petroleum ether, hexane, ethyl acetate, chloroform, and acetone), liquid N2, and diatomaceous earth were used to disrupt the cell and thus release the intracellular carotenoids. The results of this study showed that when multiple solvents were used, a synergistic effect on the extent of carotenoids recovery was obtained. Maximum concentration of total carotenoids (913 μg/L) was obtained in the treatment using liquid N2 and dimethyl sulfoxide to disrupt the cell, followed by the extraction with a solution of acetone/methanol (7:3, v/v).