Highly selective electrochemical detection of ciprofloxacin using reduced graphene oxide/poly(phenol red) modified glassy carbon electrode

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 871; p. 114254
• Main Authors: Chauhan, Ruchika, Gill, Atal A.S., Nate, Zondi, Karpoormath, Rajshekhar
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-08-2020; Elsevier Science Ltd
• Subjects: Analytical chemistry; Catalytic activity; Ciprofloxacin; Electrical impedance; Electrochemical analysis; Electrochemical determination; Electrodes; Field emission microscopy; Field emission spectroscopy; Fourier transforms; Functional groups; Glassy carbon; Graphene; Hydrogen bonding; Hydrogen storage; Nanocomposites; Oxidation; Phenol red; Phenols; Reduced graphene oxide; Selective adsorption; Sensitivity enhancement; Spectrum analysis; Sulfur trioxide; Voltammetry; Electrochemical determination; Reduced graphene oxide; Ciprofloxacin; Phenol red
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2020.114254

In this study, a novel sensor for highly selective and rapid detection of ciprofloxacin is fabricated. A facile strategy was employed to fabricate the electrode by electro-polymerization of phenol red (PPR) followed by drop-casting of reduced graphene oxide (rGO). Under optimized experimental conditions, the developed electrode shows, two linear ranges 0.002–0.05 μM and 0.05–400 μM, and the sensitivities equal to 516.41 μA μM−1 cm−2 and 0.239 μA μM−1 cm−2, respectively for ciprofloxacin detection. The rGO/PPR/GCE electrode enhanced sensitivity and low detection limit (LOD) (2 nM) of ciprofloxacin due to selective adsorption, which is accomplished by a combination of electrostatic attraction at SO3¯ sites in the PPR film, and the formation of charge assisted hydrogen bonding between ciprofloxacin and rGO surface functional groups. The novel nanocomposite was characterized by Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM), cyclic voltammetry (CV) and electrical impedance spectroscopy (EIS). Differential pulse voltammetry (DPV) was used to study the response of ciprofloxacin towards the modified electrode. The composite modified electrode displayed good electro-catalytic activity towards the oxidation of ciprofloxacin at pH 5.5 and exhibited high sensitivity and specificity towards ciprofloxacin. The practical applicability was tested in the spiked animal serum sample. The recovery of analytes in spiked samples was 97 ± 6% over the range 0.002–400 μML−1. It shows that the developed electrode can be used as a potential tool for a rapid, simple, and sensitive detection of ciprofloxacin in the serum sample.
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•Development of a novel sensor (rGO/PPR/GCE electrode) for highly selective and rapid detection of ciprofloxacin.•A new combination of rGO and poly phenol red was used for electrochemical determination of ciprofloxacin.•Simple, fast response, low detection limit, short sample preparation time, high sensitivity and selectivity.•Determination of Cfx in animal serum samples.•Good potential for biological sample and clinical analysis of Cfx.