Effect of dietary lipid level on fatty acid β-oxidation and lipid composition in various tissues of haddock, Melanogrammus aeglefinus L

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 135; no. 1; pp. 95 - 108
• Main Authors: Nanton, Dominic A., Lall, Santosh P., Ross, Neil W., McNiven, Mary A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01-05-2003
• Subjects: Animal Feed - analysis; Animals; Carbon Radioisotopes; Catabolism; Dietary Fats - pharmacology; Fatty acid; Fatty Acids - analysis; Fatty Acids - metabolism; Fish; Fishes - blood; Fishes - growth & development; Fishes - metabolism; Haddock; Lipid; Lipid Metabolism; Lipids - chemistry; Lipoproteins, VLDL - blood; Marine; Melanogrammus aeglefinus; Mitochondria, Liver - chemistry; Mitochondria, Liver - metabolism; Muscles - chemistry; Muscles - metabolism; Myocardium - chemistry; Myocardium - metabolism; Organ Specificity; Oxidation-Reduction - drug effects; Palmitoyl Coenzyme A - pharmacology; β-Oxidation; Fish; Haddock; Catabolism; β-Oxidation; Fatty acid; Lipid
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/S1096-4959(03)00052-6

Haddock (
Melanogrammus aeglefinus) is a gadoid fish species that deposits dietary lipid mainly in the liver. The fatty acid (FA)
β-oxidation activity of various tissues was evaluated in juvenile haddock fed graded levels of lipid. The catabolism of a radiolabelled FA, [1-
14C]palmitoyl-CoA, through peroxisomal and mitochondrial
β-oxidation was determined in the liver, red and white muscle of juvenile haddock fed 12, 18 and 24% lipid in the diet. There was no significant increase in the mitochondrial or peroxisomal
β-oxidation activity in the tissues tested as the dietary lipid level increased from 12 to 24%. Peroxisomes accounted for 100% of the
β-oxidation observed in the liver, whereas mitochondrial
β-oxidation dominated in the red (91%) and white muscle (97%) of juvenile haddock. Of the tissues tested, red muscle possessed the highest specific activity for
β-oxidation expressed on a per mg protein or per g wet weight basis. However, white muscle, which forms over 50% of the body mass in gadoid fish was the most important tissue in juvenile haddock for overall FA catabolism. The total lipid and FA composition of these tissues were also determined. This study confirmed that the liver was the major lipid storage organ in haddock. The hepatosomatic index (HSI; 10.0–15.2%) and lipid (73.8–79.3% wet wt.) in the liver increased significantly as dietary lipid was increased from 12 to 24% lipid. There was no significant increase in the lipid composition of the white muscle (0.8% wet wt.), red muscle (1.9% wet wt.) or heart (2.5% wet wt.).