Methanol Extracts LPS from Deep Rough Bacteria

Several rough strains ofEscherichia coli, Salmonella entericaandProteus mirabiliswere cultivated in the presence of (14C)acetate, which incorporated into their phospholipids and lipopolysaccharide. Phospholipids were removed from the cells with ethanol extraction. However, as shown by thin layer chr...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 219; no. 2; pp. 441 - 444
Main Authors:	Nurminen, Marjatta, Vaara, Martti
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 15-02-1996
Subjects:	Chromatography, Thin Layer Escherichia coli - chemistry Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - immunology Genotype Lipopolysaccharides - isolation & purification Methanol Proteus mirabilis - chemistry Proteus mirabilis - genetics Proteus mirabilis - growth & development Proteus mirabilis - immunology Salmonella - chemistry Salmonella - genetics Salmonella - growth & development Salmonella - immunology Solvents Species Specificity
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Summary:	Several rough strains ofEscherichia coli, Salmonella entericaandProteus mirabiliswere cultivated in the presence of (14C)acetate, which incorporated into their phospholipids and lipopolysaccharide. Phospholipids were removed from the cells with ethanol extraction. However, as shown by thin layer chromatography, methanol additionally extracted remarkable quantities of lipopolysaccharide from deep rough strains, but not from bacteria containing complete or nearly complete core.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1996.0252